How safe is germinal vesicle stage oocyte rescue? Aneuploidy analysis of in vitro matured oocytes

Abstract Objective To evaluate the rate and type of aneuploidies of chromosomes 13, 16, 18, 21 and 22, with respect to the length of in vitro maturation (IVM) period, and to compare the results to previously published studies on aneuploidy rates of unfertilized, uninseminated mature oocytes and firs...

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Bibliographic Details
Published in:European journal of obstetrics & gynecology and reproductive biology 2007-10, Vol.134 (2), p.213-219
Main Authors: Vlaisavljević, Veljko, Križančić Bombek, Lidija, Vokač, Nadja Kokalj, Kovačič, Borut, Čižek-Sajko, Mojca
Format: Article
Language:English
Subjects:
Adult
Aneuploidy
Biological and medical sciences
Chromosome aberrations
Chromosomes, Human - genetics
Female
Fertilization in Vitro - methods
FISH
GV-oocyte
Gynecology. Andrology. Obstetrics
Humans
In Situ Hybridization, Fluorescence
In vitro maturation
Infertility, Female - therapy
Medical genetics
Medical sciences
Obstetrics and Gynecology
Oocyte Retrieval - methods
Oocytes - growth & development
Ovulation Induction - methods
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Summary:Abstract Objective To evaluate the rate and type of aneuploidies of chromosomes 13, 16, 18, 21 and 22, with respect to the length of in vitro maturation (IVM) period, and to compare the results to previously published studies on aneuploidy rates of unfertilized, uninseminated mature oocytes and first polar bodies. Study design Two hundred and twelve immature germinal vesicle stage oocytes were assigned to two groups. After successful IVM, depending on their maturational period of 24 h (Group A) or 36 h (Group B), chromosomal analysis was performed by five color fluorescence in situ hybridization (FISH). In Groups A and B the rates of aneuploid oocytes were calculated and compared by chi-square test. Also the rates of hyperhaploidy, hypohaploidy, disomy and nullisomy were determined and compared by chi-square test. The difference was considered statistically significant at p -value of
ISSN:0301-2115
1872-7654
DOI:10.1016/j.ejogrb.2007.03.013