Reducing blood pressure in SHR with enalapril provokes redistribution of NHE3, NaPi2, and NCC and decreases NaPi2 and ACE abundance

• Published in: American journal of physiology. Renal physiology 2007-10, Vol.293 (4), p.F1197-F1208
• Main Authors: Yang, Li E, Leong, Patrick K K, McDonough, Alicia A
• Format: Article
• Language: English
• Subjects: ACE inhibitors; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Angiotensin-Converting Enzyme Inhibitors - therapeutic use; Animals; Antihypertensive Agents - pharmacology; Antihypertensive Agents - therapeutic use; Blood pressure; Blood Pressure - drug effects; Drug Therapy, Combination; Enalapril - pharmacology; Enalapril - therapeutic use; Hydralazine - pharmacology; Hydralazine - therapeutic use; Hydrochlorothiazide - pharmacology; Hydrochlorothiazide - therapeutic use; Hypertension; Hypertension - drug therapy; Hypertension - metabolism; Hypertension - pathology; Kidney Cortex - metabolism; Kidney Cortex - pathology; Kidney Medulla - metabolism; Kidney Medulla - pathology; Kidneys; Male; Peptidyl-Dipeptidase A - metabolism; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Rats, Sprague-Dawley; Reserpine - pharmacology; Reserpine - therapeutic use; Sodium; Sodium - metabolism; Sodium Chloride Symporters - metabolism; Sodium-Hydrogen Exchanger 3; Sodium-Hydrogen Exchangers - metabolism; Sodium-Phosphate Cotransporter Proteins, Type II - metabolism; Sodium-Potassium-Chloride Symporters - metabolism
• ISSN: 1931-857X; 1522-1466
• DOI: 10.1152/ajprenal.00040.2007

To determine the effects of long-term angiotensin-converting enzyme inhibition (ACEI) and blood pressure (BP) lowering on renal sodium transporter abundance and distribution in spontaneously hypertensive rats (SHR), 9-wk SHR were treated with enalapril (30 mg.kg(-1).day(-1)) for 4 wk. BP decreased from 156 +/- 4 to 96 +/- 8 mmHg. Na(+)/H(+) exchanger isoform 3 (NHE3) and Na(+)-P(i) cotransporter type 2 (NaPi2) localized to the body of the microvilli (MV) in normotensive rat strains. In untreated SHR, NHE3 partially retracted from the body to base of the MV and NaPi2 retracted to subapical vesicles. After enalapril treatment of SHR, NHE3 fully retracted to the base of the MV and, by density gradient fractionation, NHE3, NaPi2, dipeptidyl peptidase IV, myosin VI, Na-Cl cotransporter, and cortical Na-K-Cl cotransporter redistributed from low-density (apical enriched) to high-density (endosome enriched) membranes. Enalapril decreased total abundance of myosin VI (to 0.51 +/- 0.18 of untreated), ACE (0.67 +/- 0.22), and cortical NaPi2 (0.83 +/- 0.10). Normalizing SHR BP with HRH (7.5 mg/day hydralazine, 0.15 mg/day reserpine, and 3 mg/day hydrochlorothiazide) did not change Na(+) transporter density distribution or abundance. We conclude that lowering BP to normal levels in SHR does not normalize Na(+) transporter distribution, rather, chronic ACEI treatment provokes retraction of Na(+) transporters and associated proteins from transport-relevant domains of apical membranes and/or reduces their abundance.