Quantitative analysis of biofilm EPS uronic acid content

The uronic acids assay was evaluated for its ability to measure the amount of uronic acids contained within a biofilm exopolysaccharide matrix. Cytophaga lytica, a marine bacterium isolated from a naturally occurring biofilm, was used to form single-species biofilms for the method assessment. The as...

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Bibliographic Details
Published in:Journal of microbiological methods 2007-10, Vol.71 (1), p.61-65
Main Authors: Mojica, Kristina, Elsey, Danielle, Cooney, Michael J.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biofilm
Biofilms - drug effects
Biofilms - growth & development
Biological and medical sciences
Biological Assay
Cytophaga
Cytophaga - growth & development
Cytophaga - physiology
Exopolysaccharide
Extracellular Matrix - chemistry
Fundamental and applied biological sciences. Psychology
m-hydroxydiphenyl
Marine Biology
Metabolism. Enzymes
Microbiology
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - metabolism
Uronic acid
Uronic Acids - metabolism
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Summary:The uronic acids assay was evaluated for its ability to measure the amount of uronic acids contained within a biofilm exopolysaccharide matrix. Cytophaga lytica, a marine bacterium isolated from a naturally occurring biofilm, was used to form single-species biofilms for the method assessment. The assay was found to be simple, reproducible, and sensitive to 1 μg levels, suggesting its potential for application as a screening technique for compounds that inhibit the production of microbial biofilm exopolysaccharide containing uronic acids.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2007.07.010