Electrophysical characteristics of Azospirillum brasilense Sp245 during interaction with antibodies to various cell surface epitopes

This work was undertaken to examine the electrooptical characteristics of cells of Azospirillum brasilense Sp245 during their interaction with antibodies developed to various cell surface epitopes. We used the dependences of the cell suspension optical density changes induced by electroorientation o...

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Bibliographic Details
Published in:Analytical biochemistry 2007-11, Vol.370 (2), p.201-205
Main Authors: Guliy, Olga I., Matora, Larisa Y., Burygin, Gennady L., Dykman, Lev A., Ostudin, Nikolai A., Bunin, Viktor D., Ignatov, Vladimir V., Ignatov, Oleg V.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - immunology
Antibody
Antigens, Bacterial - immunology
Azospirillum brasilense - immunology
Azospirillum brasilense - physiology
Azospirillum brasilense - ultrastructure
Azospirillum brasilense Sp245
Detection of microorganisms
Electrophysical property
Electrophysiology
Epitopes - immunology
Lipopolysaccharides - immunology
Microscopy, Electron
O Antigens - immunology
Rabbits
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Summary:This work was undertaken to examine the electrooptical characteristics of cells of Azospirillum brasilense Sp245 during their interaction with antibodies developed to various cell surface epitopes. We used the dependences of the cell suspension optical density changes induced by electroorientation on the orienting field frequency (740, 1000, 1450, 2000, and 2800 kHz). Cell interactions with homologous strain-specific antibodies to the A. brasilense Sp245 O antigen and with homologous antibodies to whole bacterial cells brought about considerable changes in the electrooptical properties of the bacterial suspension. When genus-specific antibodies to the flagellin of the Azospirillum sheathed flagellum and antibodies to the serologically distinct O antigen of A. brasilense Sp7 were included in the A. brasilense Sp245 suspension, the changes caused in the electrooptical signal were slight and had values close to those for the above changes. These findings agree well with the immunochemical characteristics of the Azospirillum O antigens and with the data on the topographical distribution of the Azospirillum major cell surface antigens. The obtained results can serve as a basis for the development of a rapid test for the intraspecies detection of microorganisms.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2007.07.013