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Electrophysical characteristics of Azospirillum brasilense Sp245 during interaction with antibodies to various cell surface epitopes
This work was undertaken to examine the electrooptical characteristics of cells of Azospirillum brasilense Sp245 during their interaction with antibodies developed to various cell surface epitopes. We used the dependences of the cell suspension optical density changes induced by electroorientation o...
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| Published in: | Analytical biochemistry 2007-11, Vol.370 (2), p.201-205 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
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| cited_by | cdi_FETCH-LOGICAL-c348t-69b89682e7a1ea2d026bab3df5519e29a5f2aa699d7bfb303bb3568810967f463 |
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| cites | cdi_FETCH-LOGICAL-c348t-69b89682e7a1ea2d026bab3df5519e29a5f2aa699d7bfb303bb3568810967f463 |
| container_end_page | 205 |
| container_issue | 2 |
| container_start_page | 201 |
| container_title | Analytical biochemistry |
| container_volume | 370 |
| creator | Guliy, Olga I. Matora, Larisa Y. Burygin, Gennady L. Dykman, Lev A. Ostudin, Nikolai A. Bunin, Viktor D. Ignatov, Vladimir V. Ignatov, Oleg V. |
| description | This work was undertaken to examine the electrooptical characteristics of cells of
Azospirillum brasilense Sp245 during their interaction with antibodies developed to various cell surface epitopes. We used the dependences of the cell suspension optical density changes induced by electroorientation on the orienting field frequency (740, 1000, 1450, 2000, and 2800
kHz). Cell interactions with homologous strain-specific antibodies to the
A. brasilense Sp245 O antigen and with homologous antibodies to whole bacterial cells brought about considerable changes in the electrooptical properties of the bacterial suspension. When genus-specific antibodies to the flagellin of the
Azospirillum sheathed flagellum and antibodies to the serologically distinct O antigen of
A. brasilense Sp7 were included in the
A. brasilense Sp245 suspension, the changes caused in the electrooptical signal were slight and had values close to those for the above changes. These findings agree well with the immunochemical characteristics of the
Azospirillum O antigens and with the data on the topographical distribution of the
Azospirillum major cell surface antigens. The obtained results can serve as a basis for the development of a rapid test for the intraspecies detection of microorganisms. |
| doi_str_mv | 10.1016/j.ab.2007.07.013 |
| format | article |
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Azospirillum brasilense Sp245 during their interaction with antibodies developed to various cell surface epitopes. We used the dependences of the cell suspension optical density changes induced by electroorientation on the orienting field frequency (740, 1000, 1450, 2000, and 2800
kHz). Cell interactions with homologous strain-specific antibodies to the
A. brasilense Sp245 O antigen and with homologous antibodies to whole bacterial cells brought about considerable changes in the electrooptical properties of the bacterial suspension. When genus-specific antibodies to the flagellin of the
Azospirillum sheathed flagellum and antibodies to the serologically distinct O antigen of
A. brasilense Sp7 were included in the
A. brasilense Sp245 suspension, the changes caused in the electrooptical signal were slight and had values close to those for the above changes. These findings agree well with the immunochemical characteristics of the
Azospirillum O antigens and with the data on the topographical distribution of the
Azospirillum major cell surface antigens. The obtained results can serve as a basis for the development of a rapid test for the intraspecies detection of microorganisms.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2007.07.013</identifier><identifier>PMID: 17723223</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antibodies, Bacterial - immunology ; Antibody ; Antigens, Bacterial - immunology ; Azospirillum brasilense - immunology ; Azospirillum brasilense - physiology ; Azospirillum brasilense - ultrastructure ; Azospirillum brasilense Sp245 ; Detection of microorganisms ; Electrophysical property ; Electrophysiology ; Epitopes - immunology ; Lipopolysaccharides - immunology ; Microscopy, Electron ; O Antigens - immunology ; Rabbits</subject><ispartof>Analytical biochemistry, 2007-11, Vol.370 (2), p.201-205</ispartof><rights>2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-69b89682e7a1ea2d026bab3df5519e29a5f2aa699d7bfb303bb3568810967f463</citedby><cites>FETCH-LOGICAL-c348t-69b89682e7a1ea2d026bab3df5519e29a5f2aa699d7bfb303bb3568810967f463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17723223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guliy, Olga I.</creatorcontrib><creatorcontrib>Matora, Larisa Y.</creatorcontrib><creatorcontrib>Burygin, Gennady L.</creatorcontrib><creatorcontrib>Dykman, Lev A.</creatorcontrib><creatorcontrib>Ostudin, Nikolai A.</creatorcontrib><creatorcontrib>Bunin, Viktor D.</creatorcontrib><creatorcontrib>Ignatov, Vladimir V.</creatorcontrib><creatorcontrib>Ignatov, Oleg V.</creatorcontrib><title>Electrophysical characteristics of Azospirillum brasilense Sp245 during interaction with antibodies to various cell surface epitopes</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>This work was undertaken to examine the electrooptical characteristics of cells of
Azospirillum brasilense Sp245 during their interaction with antibodies developed to various cell surface epitopes. We used the dependences of the cell suspension optical density changes induced by electroorientation on the orienting field frequency (740, 1000, 1450, 2000, and 2800
kHz). Cell interactions with homologous strain-specific antibodies to the
A. brasilense Sp245 O antigen and with homologous antibodies to whole bacterial cells brought about considerable changes in the electrooptical properties of the bacterial suspension. When genus-specific antibodies to the flagellin of the
Azospirillum sheathed flagellum and antibodies to the serologically distinct O antigen of
A. brasilense Sp7 were included in the
A. brasilense Sp245 suspension, the changes caused in the electrooptical signal were slight and had values close to those for the above changes. These findings agree well with the immunochemical characteristics of the
Azospirillum O antigens and with the data on the topographical distribution of the
Azospirillum major cell surface antigens. The obtained results can serve as a basis for the development of a rapid test for the intraspecies detection of microorganisms.</description><subject>Animals</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Antibody</subject><subject>Antigens, Bacterial - immunology</subject><subject>Azospirillum brasilense - immunology</subject><subject>Azospirillum brasilense - physiology</subject><subject>Azospirillum brasilense - ultrastructure</subject><subject>Azospirillum brasilense Sp245</subject><subject>Detection of microorganisms</subject><subject>Electrophysical property</subject><subject>Electrophysiology</subject><subject>Epitopes - immunology</subject><subject>Lipopolysaccharides - immunology</subject><subject>Microscopy, Electron</subject><subject>O Antigens - immunology</subject><subject>Rabbits</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp1kc1r3DAQxUVpabZp7z0VnXrzVh9r2e4thPQDAj2kOQtJHndn8VquRk5Jzv3DI7MLPRUG5vLe481vGHsvxVYKaT4dts5vlRDNdh2pX7CNFJ2phBbdS7YRQuhKma65YG-IDkJIuavNa3Yhm0ZppfSG_b0ZIeQU5_0jYXAjD3uXXMiQkDIG4nHgV0-RZkw4jsuR--QIR5gI-N2sdjXvl4TTL45T8RQjxon_wbznbsroY49APEf-4BLGhXiAceS0pMEF4DBjjjPQW_ZqcCPBu_O-ZPdfbn5ef6tuf3z9fn11WwW9a3NlOt92plXQOAlO9UIZ77zuh7qWHajO1YNyznRd3_jBa6G917Vp2xVJM-yMvmQfT7lzir8XoGyPSGsjN0EpZ02rC59aFaE4CUOKRAkGOyc8uvRopbAreXuwztuVvF1H6mL5cM5e_BH6f4Yz6iL4fBJAufABIVkKCFOAHlN5ge0j_j_9GW9mlgc</recordid><startdate>20071115</startdate><enddate>20071115</enddate><creator>Guliy, Olga I.</creator><creator>Matora, Larisa Y.</creator><creator>Burygin, Gennady L.</creator><creator>Dykman, Lev A.</creator><creator>Ostudin, Nikolai A.</creator><creator>Bunin, Viktor D.</creator><creator>Ignatov, Vladimir V.</creator><creator>Ignatov, Oleg V.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071115</creationdate><title>Electrophysical characteristics of Azospirillum brasilense Sp245 during interaction with antibodies to various cell surface epitopes</title><author>Guliy, Olga I. ; Matora, Larisa Y. ; Burygin, Gennady L. ; Dykman, Lev A. ; Ostudin, Nikolai A. ; Bunin, Viktor D. ; Ignatov, Vladimir V. ; Ignatov, Oleg V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-69b89682e7a1ea2d026bab3df5519e29a5f2aa699d7bfb303bb3568810967f463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Antibody</topic><topic>Antigens, Bacterial - immunology</topic><topic>Azospirillum brasilense - immunology</topic><topic>Azospirillum brasilense - physiology</topic><topic>Azospirillum brasilense - ultrastructure</topic><topic>Azospirillum brasilense Sp245</topic><topic>Detection of microorganisms</topic><topic>Electrophysical property</topic><topic>Electrophysiology</topic><topic>Epitopes - immunology</topic><topic>Lipopolysaccharides - immunology</topic><topic>Microscopy, Electron</topic><topic>O Antigens - immunology</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guliy, Olga I.</creatorcontrib><creatorcontrib>Matora, Larisa Y.</creatorcontrib><creatorcontrib>Burygin, Gennady L.</creatorcontrib><creatorcontrib>Dykman, Lev A.</creatorcontrib><creatorcontrib>Ostudin, Nikolai A.</creatorcontrib><creatorcontrib>Bunin, Viktor D.</creatorcontrib><creatorcontrib>Ignatov, Vladimir V.</creatorcontrib><creatorcontrib>Ignatov, Oleg V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guliy, Olga I.</au><au>Matora, Larisa Y.</au><au>Burygin, Gennady L.</au><au>Dykman, Lev A.</au><au>Ostudin, Nikolai A.</au><au>Bunin, Viktor D.</au><au>Ignatov, Vladimir V.</au><au>Ignatov, Oleg V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrophysical characteristics of Azospirillum brasilense Sp245 during interaction with antibodies to various cell surface epitopes</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2007-11-15</date><risdate>2007</risdate><volume>370</volume><issue>2</issue><spage>201</spage><epage>205</epage><pages>201-205</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>This work was undertaken to examine the electrooptical characteristics of cells of
Azospirillum brasilense Sp245 during their interaction with antibodies developed to various cell surface epitopes. We used the dependences of the cell suspension optical density changes induced by electroorientation on the orienting field frequency (740, 1000, 1450, 2000, and 2800
kHz). Cell interactions with homologous strain-specific antibodies to the
A. brasilense Sp245 O antigen and with homologous antibodies to whole bacterial cells brought about considerable changes in the electrooptical properties of the bacterial suspension. When genus-specific antibodies to the flagellin of the
Azospirillum sheathed flagellum and antibodies to the serologically distinct O antigen of
A. brasilense Sp7 were included in the
A. brasilense Sp245 suspension, the changes caused in the electrooptical signal were slight and had values close to those for the above changes. These findings agree well with the immunochemical characteristics of the
Azospirillum O antigens and with the data on the topographical distribution of the
Azospirillum major cell surface antigens. The obtained results can serve as a basis for the development of a rapid test for the intraspecies detection of microorganisms.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17723223</pmid><doi>10.1016/j.ab.2007.07.013</doi><tpages>5</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 2007-11, Vol.370 (2), p.201-205 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Antibodies, Bacterial - immunology Antibody Antigens, Bacterial - immunology Azospirillum brasilense - immunology Azospirillum brasilense - physiology Azospirillum brasilense - ultrastructure Azospirillum brasilense Sp245 Detection of microorganisms Electrophysical property Electrophysiology Epitopes - immunology Lipopolysaccharides - immunology Microscopy, Electron O Antigens - immunology Rabbits |
| title | Electrophysical characteristics of Azospirillum brasilense Sp245 during interaction with antibodies to various cell surface epitopes |
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