Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes

Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extrac...

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Bibliographic Details
Published in:Parasitology 2007-11, Vol.134 (12), p.1831-1838
Main Authors: STEPEK, G., CURTIS, R. H. C., KERRY, B. R., SHEWRY, P. R., CLARK, S. J., LOWE, A. E., DUCE, I. R., BUTTLE, D. J., BEHNKE, J. M.
Format: Article
Language:English
Subjects:
Actinidia - chemistry
Actinidia - enzymology
Ananas - chemistry
Ananas - enzymology
Animals
Antinematodal Agents - pharmacology
Biological and medical sciences
botanical pesticides
Carica - chemistry
Carica - enzymology
Cysteine Endopeptidases - pharmacology
Cysteine Proteinase Inhibitors - pharmacology
cysteine proteinases
Female
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Globodera rostochiensis
in vitro
Invertebrates
Leucine - analogs & derivatives
Leucine - pharmacology
Magnoliopsida - chemistry
Magnoliopsida - enzymology
Magnoliopsida - parasitology
mechanism of action
Meloidogyne incognita
Meloidogyne javanica
Meloidogyne spp
Microscopy, Electron, Scanning
nematicidal properties
nematicide
nematode control
nematode larvae
plant cysteine proteinases
plant extracts
Plant Extracts - pharmacology
plant parasitic nematodes
Time Factors
Tylenchoidea - drug effects
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Summary:Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nematodes. The specific inhibitor of cysteine proteinases, E-64, blocked this activity completely, indicating that it was essentially mediated by cysteine proteinases. In vitro, plant cysteine proteinases are active against second-stage juveniles of M. incognita and M. javanica, and some cysteine proteinases also affect the second-stage juveniles of Globodera rostochiensis. It is not known yet whether these plant extracts will interfere with, or prevent invasion of, host plants.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182007003289