Investigating the Kinetics of the Enzymatic Depolymerization of Polygalacturonic Acid in Continuous UF-Membrane Reactors

A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention tim...

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Bibliographic Details
Published in:Biotechnology progress 2007-09, Vol.23 (5), p.1111-1117
Main Authors: Gallifuoco, A, Cantarella, M, Marucci, M
Format: Article
Language:English
Subjects:
Aspergillus - enzymology
Aspergillus japonicus
Biological and medical sciences
Bioreactors
Biotechnology
Computer Simulation
enzymatic hydrolysis
enzyme activity
enzyme kinetics
Enzymes, Immobilized - chemistry
Fundamental and applied biological sciences. Psychology
Hydrolysis
Kinetics
Membranes, Artificial
Models, Chemical
Pectins - metabolism
polygalacturonase
Polygalacturonase - metabolism
polygalacturonic acid
Polymers - chemistry
polyuronides
ultrafiltration membrane reactors
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Summary:A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention time (from 101 to 142 min) and membrane molecular weight cutoff (from 1 to 30 kDa) on the rate of permeable oligomers production is investigated. Reaction products are clustered in two different classes, those sized below the membrane cutoff and those above. The reducing power measured in the permeate is used as an estimate of total product concentration. The characteristic breakdown times range from 40 to 100 min. The overall kinetics obeys a first‐order law with a characteristic time estimated to 24 min. New mathematical data handling are developed and illustrated using the experimental data obtained. Finally, the body of the experimental results suggests useful indications (reactor productivity, breakdown induction period) for implementing the bioprocess at the industrial scale.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp070177y