Rhizobium selenireducens sp. nov.: A Selenite-Reducing α-Proteobacteria Isolated From a Bioreactor

A Gram-negative, nonpigmented bacterium designated strain B1 was isolated from a laboratory bioreactor that reduced selenate to elemental red selenium (Se⁰). 16S rRNA gene-sequence alignment identified the isolate as a Rhizobium sp. belonging to the Rhizobium clade, which includes R. daejeonense, R....

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Bibliographic Details
Published in:Current microbiology 2007-11, Vol.55 (5), p.455-460
Main Authors: Hunter, W. J, Kuykendall, L. D, Manter, D. K
Format: Article
Language:English
Subjects:
Alphaproteobacteria - genetics
Alphaproteobacteria - isolation & purification
Alphaproteobacteria - metabolism
bacteria
Base Composition - genetics
bioreactors
Bioreactors - microbiology
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
fatty acids
Fatty Acids - metabolism
Molecular Sequence Data
Phylogeny
Rhizobium
Rhizobium - classification
Rhizobium - genetics
Rhizobium - metabolism
ribosomal RNA
RNA, Ribosomal, 16S - genetics
Selenic Acid
selenium
Selenium - metabolism
Selenium Compounds - metabolism
Sequence Analysis, DNA
variance
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Summary:A Gram-negative, nonpigmented bacterium designated strain B1 was isolated from a laboratory bioreactor that reduced selenate to elemental red selenium (Se⁰). 16S rRNA gene-sequence alignment identified the isolate as a Rhizobium sp. belonging to the Rhizobium clade, which includes R. daejeonense, R. giardinii, R. undicola, R. larrymoorei, R. radiobacter, R. rubi, and R. vitis. R. radiobacter and R. rubi are its closest relatives as indicated by 16S rRNA gene-sequence alignments, which differ from strain B1 by 2.6% and 2.8%, respectively. Within this group, strains that show variances > 0.8% to 2.2% have been classified as different species. The major cellular fatty acids present in the B1 strain were C16:0 (1.8%), C18:0 (3.38%), 18:0 3-OH (1.6%), 18:1 ω7c (86.8%), 19:0 cycloω8c (1.5%), and summed features 2 (3.8%) and 3 (1.2%). The large amount of 18:1 ω7c present is constant with members of this group of bacteria, but the small amounts of 16:0, 19:0 cycloω8c, and summed feature 3 shows variance from R. radiobacter and R. rubi. The strain's phenotypic and biochemical characteristics are consistent with its placement in this genus.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-007-9020-9