Gene expression analysis of estrogenic compounds in the liver of common carp (Cyprinus carpio) using a custom cDNA microarray

Exposure to a variety of compounds with estrogenic activity has been shown to interfere with normal developmental and reproductive processes in various vertebrate species. The aim of this study was to determine the transcriptional profile of the natural estrogen, 17 beta‐estradiol, and three synthet...

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Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2007-01, Vol.21 (5), p.299-311
Main Authors: Moens, Lotte N., van der Ven, K., Van Remortel, P., Del-Favero, J., De Coen, W. M.
Format: Article
Language:English
Subjects:
Animals
Carps - genetics
Cluster Analysis
Common Carp
Cyprinus carpio
Dose-Response Relationship, Drug
Endocrine Disruption
Estrogens - pharmacology
Gene Expression
Gene Expression Profiling - methods
Gene Expression Regulation - drug effects
Liver - drug effects
Liver - metabolism
Microarray
Oligonucleotide Array Sequence Analysis - methods
Time Factors
Vitellogenins - genetics
Vitellogenins - metabolism
Xenoestrogens
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Summary:Exposure to a variety of compounds with estrogenic activity has been shown to interfere with normal developmental and reproductive processes in various vertebrate species. The aim of this study was to determine the transcriptional profile of the natural estrogen, 17 beta‐estradiol, and three synthetic estrogenic compounds (4‐nonylphenol, bisphenol A, ethinylestradiol) in the liver of common carp, using a custom cDNA microarray. For that purpose, fish were aqueously exposed to three concentrations of each chemical for 24 or 96 h. Microarray analysis revealed that a total of 185 different gene transcripts were differentially expressed following exposure to at least one of the estrogen(‐like) concentrations. We were able to identify a common set of 28 gene fragments, whose expression was significantly modified in the same way by the three xenoestrogens and 17 beta‐estradiol. Although several of these gene expression effects corroborated past literature data, we also discovered some novel target genes of (xeno)estrogen exposure, providing interesting insights into the molecular basis of estrogenic effects. In addition, each of the four compounds induced gene expression changes that were not, or only partially, shared by the other chemicals, suggesting that not all chemicals with estrogenic activity act alike. These results demonstrate the potential of our custom Cyprinus carpio microarray to detect common estrogen‐like activity as well as to identify unique compound‐associated effects of (estrogenic) endocrine disruptors in fish. © 2007 Wiley Periodicals, Inc. J Biochem Mol Toxicol 21:299–311, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20190
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.20190