EPR spectroscopy and theoretical study of γ-irradiated asparagine and aspartic acid in solid state

Aspartic acid (Asp) and asparagine (Asn) are vulnerable amino acids. One-electron addition or withdrawal reactions initiate many deleterious processes involving these amino acids. To study these redox processes we have irradiated by γ-rays asparagine or aspartic acid in the solid state. The nature o...

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Bibliographic Details
Published in:Biophysical chemistry 2007, Vol.125 (1), p.92-103
Main Authors: Strzelczak, Grazyna, Bergès, Jacqueline, Houée-Levin, Chantal, Pogocki, Dariusz, Bobrowski, Krzysztof
Format: Article
Language:English
Subjects:
Asparagine
Asparagine - chemistry
Asparagine - radiation effects
Aspartic acid
Aspartic Acid - chemistry
Aspartic Acid - radiation effects
Decarboxylation
DFT calculations
Electron paramagnetic resonance (EPR)
Electron Spin Resonance Spectroscopy
Free Radicals - chemistry
Gamma Rays
Molecular Conformation - radiation effects
γ-Radiolysis
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Summary:Aspartic acid (Asp) and asparagine (Asn) are vulnerable amino acids. One-electron addition or withdrawal reactions initiate many deleterious processes involving these amino acids. To study these redox processes we have irradiated by γ-rays asparagine or aspartic acid in the solid state. The nature of the resulting free radicals was determined by electron paramagnetic resonance (EPR) and by calculations using DFT methods in various environments. Reactions initiated by electron transfer are different for both amino acids: Asn anion loses hydrogen atom whereas the cation undergoes decarboxylation. Conversely, Asp cation loses hydrogen atom from amine group, which triggers decarboxylation.
ISSN:0301-4622
1873-4200
DOI:10.1016/j.bpc.2006.06.017