Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine

A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed...

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Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2007/10/01, Vol.30(10), pp.1943-1946
Main Authors: Samejima, Keijiro, Otani, Masahiro, Murakami, Yasuko, Oka, Takami, Kasai, Misao, Tsumoto, Hiroki, Kohda, Kohfuku
Format: Article
Language:English
Subjects:
Cell Line
Cells - chemistry
Chromatography, High Pressure Liquid
cultured mammalian cell
Cyclohexylamines - pharmacology
electrospray ionization
Enzyme Inhibitors - pharmacology
heptafluorobutyryl derivative
Humans
Mass Spectrometry
polyamine
Proteins - chemistry
Proteins - isolation & purification
Reference Standards
Spectrometry, Mass, Electrospray Ionization
Spermidine - analysis
Spermine - analysis
stable isotope labeled polyamine
time-of-flight mass spectrometry
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Summary:A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15N-labeled polyamines.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.30.1943