Loading…

Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine

A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed...

Full description

Saved in:

Bibliographic Details
Published in:	Biological & Pharmaceutical Bulletin 2007/10/01, Vol.30(10), pp.1943-1946
Main Authors:	Samejima, Keijiro, Otani, Masahiro, Murakami, Yasuko, Oka, Takami, Kasai, Misao, Tsumoto, Hiroki, Kohda, Kohfuku
Format:	Article
Language:	English
Subjects:	Cell Line Cells - chemistry Chromatography, High Pressure Liquid cultured mammalian cell Cyclohexylamines - pharmacology electrospray ionization Enzyme Inhibitors - pharmacology heptafluorobutyryl derivative Humans Mass Spectrometry polyamine Proteins - chemistry Proteins - isolation & purification Reference Standards Spectrometry, Mass, Electrospray Ionization Spermidine - analysis Spermine - analysis stable isotope labeled polyamine time-of-flight mass spectrometry
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c690t-3c84d486bc763b8674a9b054d7f97a420a02248e1472f511ab8d1e63a37b19713
cites	cdi_FETCH-LOGICAL-c690t-3c84d486bc763b8674a9b054d7f97a420a02248e1472f511ab8d1e63a37b19713
container_end_page	1946
container_issue	10
container_start_page	1943
container_title	Biological & Pharmaceutical Bulletin
container_volume	30
creator	Samejima, Keijiro Otani, Masahiro Murakami, Yasuko Oka, Takami Kasai, Misao Tsumoto, Hiroki Kohda, Kohfuku
description	A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15N-labeled polyamines.
doi_str_mv	10.1248/bpb.30.1943
format	article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68356367</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3121388971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c690t-3c84d486bc763b8674a9b054d7f97a420a02248e1472f511ab8d1e63a37b19713</originalsourceid><addsrcrecordid>eNpdkU1v1DAQhi0EokvhxB1FQuKCUvwVOzlC6dJKrTi0nC3HmXS9cuzFdg7lwG_Hu1laqZexR_PMO6N5EXpP8BmhvP3S7_ozVv4dZy_QijAu64aS5iVa4Y60tSBNe4LepLTFGEtM2Wt0QmRHJBVyhf5eODA5hrSL-qG6Ct7-0dkGX2k_VHd2gjqM9drZ-02ubnRK1e3uwE-QozXVDeRNGKoxxOrWTrPL2kOYU_UdMsTJ-kUrjPu2kg_Ww0F5ST28Ra9G7RK8O76n6Nf64u78sr7--ePq_Ot1bUSHc81Mywfeit5IwfpWSK67Hjd8kGMnNadYY1pOAYRLOjaE6L4dCAimmexJJwk7RZ8W3V0Mv2dIWU02GXBu2VeJljWCCVnAj8_AbZijL7spwnnHBOuoKNTnhTLlcinCqHbRTjo-KILV3hRVTFGs_Isphf5w1Jz7CYYn9uhCAdYLUKrWaBe8K7d5mmyS7G1wQdHioMKY4TIGU36Q3wfBSMs5LULfFqFtyvoeHifpmK1x8LgVPsZD__-i2eiowLN_qBS10A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449363926</pqid></control><display><type>article</type><title>Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine</title><source>Full-Text Journals in Chemistry (Open access)</source><creator>Samejima, Keijiro ; Otani, Masahiro ; Murakami, Yasuko ; Oka, Takami ; Kasai, Misao ; Tsumoto, Hiroki ; Kohda, Kohfuku</creator><creatorcontrib>Samejima, Keijiro ; Otani, Masahiro ; Murakami, Yasuko ; Oka, Takami ; Kasai, Misao ; Tsumoto, Hiroki ; Kohda, Kohfuku ; Research Institute of Pharmaceutical Sciences ; Musashino University</creatorcontrib><description>A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15N-labeled polyamines.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.30.1943</identifier><identifier>PMID: 17917267</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Cell Line ; Cells - chemistry ; Chromatography, High Pressure Liquid ; cultured mammalian cell ; Cyclohexylamines - pharmacology ; electrospray ionization ; Enzyme Inhibitors - pharmacology ; heptafluorobutyryl derivative ; Humans ; Mass Spectrometry ; polyamine ; Proteins - chemistry ; Proteins - isolation & purification ; Reference Standards ; Spectrometry, Mass, Electrospray Ionization ; Spermidine - analysis ; Spermine - analysis ; stable isotope labeled polyamine ; time-of-flight mass spectrometry</subject><ispartof>Biological and Pharmaceutical Bulletin, 2007/10/01, Vol.30(10), pp.1943-1946</ispartof><rights>2007 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c690t-3c84d486bc763b8674a9b054d7f97a420a02248e1472f511ab8d1e63a37b19713</citedby><cites>FETCH-LOGICAL-c690t-3c84d486bc763b8674a9b054d7f97a420a02248e1472f511ab8d1e63a37b19713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17917267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Samejima, Keijiro</creatorcontrib><creatorcontrib>Otani, Masahiro</creatorcontrib><creatorcontrib>Murakami, Yasuko</creatorcontrib><creatorcontrib>Oka, Takami</creatorcontrib><creatorcontrib>Kasai, Misao</creatorcontrib><creatorcontrib>Tsumoto, Hiroki</creatorcontrib><creatorcontrib>Kohda, Kohfuku</creatorcontrib><creatorcontrib>Research Institute of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Musashino University</creatorcontrib><title>Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15N-labeled polyamines.</description><subject>Cell Line</subject><subject>Cells - chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>cultured mammalian cell</subject><subject>Cyclohexylamines - pharmacology</subject><subject>electrospray ionization</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>heptafluorobutyryl derivative</subject><subject>Humans</subject><subject>Mass Spectrometry</subject><subject>polyamine</subject><subject>Proteins - chemistry</subject><subject>Proteins - isolation & purification</subject><subject>Reference Standards</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Spermidine - analysis</subject><subject>Spermine - analysis</subject><subject>stable isotope labeled polyamine</subject><subject>time-of-flight mass spectrometry</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNpdkU1v1DAQhi0EokvhxB1FQuKCUvwVOzlC6dJKrTi0nC3HmXS9cuzFdg7lwG_Hu1laqZexR_PMO6N5EXpP8BmhvP3S7_ozVv4dZy_QijAu64aS5iVa4Y60tSBNe4LepLTFGEtM2Wt0QmRHJBVyhf5eODA5hrSL-qG6Ct7-0dkGX2k_VHd2gjqM9drZ-02ubnRK1e3uwE-QozXVDeRNGKoxxOrWTrPL2kOYU_UdMsTJ-kUrjPu2kg_Ww0F5ST28Ra9G7RK8O76n6Nf64u78sr7--ePq_Ot1bUSHc81Mywfeit5IwfpWSK67Hjd8kGMnNadYY1pOAYRLOjaE6L4dCAimmexJJwk7RZ8W3V0Mv2dIWU02GXBu2VeJljWCCVnAj8_AbZijL7spwnnHBOuoKNTnhTLlcinCqHbRTjo-KILV3hRVTFGs_Isphf5w1Jz7CYYn9uhCAdYLUKrWaBe8K7d5mmyS7G1wQdHioMKY4TIGU36Q3wfBSMs5LULfFqFtyvoeHifpmK1x8LgVPsZD__-i2eiowLN_qBS10A</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Samejima, Keijiro</creator><creator>Otani, Masahiro</creator><creator>Murakami, Yasuko</creator><creator>Oka, Takami</creator><creator>Kasai, Misao</creator><creator>Tsumoto, Hiroki</creator><creator>Kohda, Kohfuku</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine</title><author>Samejima, Keijiro ; Otani, Masahiro ; Murakami, Yasuko ; Oka, Takami ; Kasai, Misao ; Tsumoto, Hiroki ; Kohda, Kohfuku</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c690t-3c84d486bc763b8674a9b054d7f97a420a02248e1472f511ab8d1e63a37b19713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Cell Line</topic><topic>Cells - chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>cultured mammalian cell</topic><topic>Cyclohexylamines - pharmacology</topic><topic>electrospray ionization</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>heptafluorobutyryl derivative</topic><topic>Humans</topic><topic>Mass Spectrometry</topic><topic>polyamine</topic><topic>Proteins - chemistry</topic><topic>Proteins - isolation & purification</topic><topic>Reference Standards</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Spermidine - analysis</topic><topic>Spermine - analysis</topic><topic>stable isotope labeled polyamine</topic><topic>time-of-flight mass spectrometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samejima, Keijiro</creatorcontrib><creatorcontrib>Otani, Masahiro</creatorcontrib><creatorcontrib>Murakami, Yasuko</creatorcontrib><creatorcontrib>Oka, Takami</creatorcontrib><creatorcontrib>Kasai, Misao</creatorcontrib><creatorcontrib>Tsumoto, Hiroki</creatorcontrib><creatorcontrib>Kohda, Kohfuku</creatorcontrib><creatorcontrib>Research Institute of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Musashino University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Samejima, Keijiro</au><au>Otani, Masahiro</au><au>Murakami, Yasuko</au><au>Oka, Takami</au><au>Kasai, Misao</au><au>Tsumoto, Hiroki</au><au>Kohda, Kohfuku</au><aucorp>Research Institute of Pharmaceutical Sciences</aucorp><aucorp>Musashino University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine</atitle><jtitle>Biological & Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>30</volume><issue>10</issue><spage>1943</spage><epage>1946</epage><pages>1943-1946</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>A sensitive method for the determination of polyamines in mammalian cells was described using electrospray ionization and time-of-flight mass spectrometer. This method was 50-fold more sensitive than the previous method using ionspray ionization and quadrupole mass spectrometer. The method employed the partial purification and derivatization of polyamines, but allowed a measurement of multiple samples which contained picomol amounts of polyamines. Time required for data acquisition of one sample was approximately 2 min. The method was successfully applied for the determination of reduced spermidine and spermine contents in cultured cells under the inhibition of aminopropyltransferases. In addition, a new proper internal standard was proposed for the tracer experiment using 15N-labeled polyamines.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>17917267</pmid><doi>10.1248/bpb.30.1943</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext	fulltext
identifier	ISSN: 0918-6158
ispartof	Biological and Pharmaceutical Bulletin, 2007/10/01, Vol.30(10), pp.1943-1946
issn	0918-6158 1347-5215
language	eng
recordid	cdi_proquest_miscellaneous_68356367
source	Full-Text Journals in Chemistry (Open access)
subjects	Cell Line Cells - chemistry Chromatography, High Pressure Liquid cultured mammalian cell Cyclohexylamines - pharmacology electrospray ionization Enzyme Inhibitors - pharmacology heptafluorobutyryl derivative Humans Mass Spectrometry polyamine Proteins - chemistry Proteins - isolation & purification Reference Standards Spectrometry, Mass, Electrospray Ionization Spermidine - analysis Spermine - analysis stable isotope labeled polyamine time-of-flight mass spectrometry
title	Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T12%3A57%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrospray%20Ionization%20and%20Time-of-Flight%20Mass%20Spectrometric%20Method%20for%20Simultaneous%20Determination%20of%20Spermidine%20and%20Spermine&rft.jtitle=Biological%20&%20Pharmaceutical%20Bulletin&rft.au=Samejima,%20Keijiro&rft.aucorp=Research%20Institute%20of%20Pharmaceutical%20Sciences&rft.date=2007-10-01&rft.volume=30&rft.issue=10&rft.spage=1943&rft.epage=1946&rft.pages=1943-1946&rft.issn=0918-6158&rft.eissn=1347-5215&rft_id=info:doi/10.1248/bpb.30.1943&rft_dat=%3Cproquest_cross%3E3121388971%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c690t-3c84d486bc763b8674a9b054d7f97a420a02248e1472f511ab8d1e63a37b19713%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1449363926&rft_id=info:pmid/17917267&rfr_iscdi=true