In vitro exposure of human chondrocytes to pulsed electromagnetic fields

The effect of pulsed electromagnetic fields (PEMFs) on the proliferation and survival of matrix-induced autologous chondrocyte implantation (MACI)-derived cells was studied to ascertain the healing potential of PEMFs. MACI-derived cells were taken from cartilage biopsies 6 months after surgery and c...

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Bibliographic Details
Published in:European journal of histochemistry 2007-07, Vol.51 (3), p.203-212
Main Authors: Nicolin, Vanessa, Ponti, C, Baldini, G, Gibellini, D, Bortul, R, Zweyer, M, Martinelli, B, Narducci, P
Format: Article
Language:English
Subjects:
Cartilage, Articular - pathology
Cartilage, Articular - surgery
Cell Proliferation
Cell Survival - radiation effects
Cells, Cultured
Chondrocytes - pathology
Chondrocytes - radiation effects
Chondrocytes - transplantation
Electromagnetic Fields
Humans
Immunohistochemistry
Knee Injuries - pathology
Knee Injuries - surgery
Knee Joint - pathology
Knee Joint - surgery
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Summary:The effect of pulsed electromagnetic fields (PEMFs) on the proliferation and survival of matrix-induced autologous chondrocyte implantation (MACI)-derived cells was studied to ascertain the healing potential of PEMFs. MACI-derived cells were taken from cartilage biopsies 6 months after surgery and cultured. No dedifferentiation towards the fibro- blastic phenotype occurred, indicating the success of the surgical implantation. The MACI-derived cultured chondrocytes were exposed to 12 h/day (short term) or 4 h/day (long term) PEMFs exposure (magnetic field intensity, 2 mT; frequency, 75 Hz) and proliferation rate determined by flow cytometric analysis. The PEMFs exposure elicited a significant increase of cell number in the SG2M cell cycle phase. Moreover, cells isolated from MACI scaffolds showed the presence of collagen type II, a typical marker of chondrocyte functionality. The results show that MACI membranes represent an optimal bioengineering device to support chondrocyte growth and proliferation in surgical implants. The surgical implant of MACI combined with physiotherapy is suggested as a promising approach for a faster and safer treatment of cartilage traumatic lesions.
ISSN:1121-760X
2038-8306