Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis

A Scopulariopsis brevicaulis poultry farm isolate was chosen to study factors influencing keratinase production. The parameters were optimized by factorial design. The highest enzyme production by this fungus was obtained at pH 7.5, a temperature of 30 °C and a growth period of 5 weeks. The producti...

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Bibliographic Details
Published in:Bioresource technology 2007-04, Vol.98 (6), p.1298-1303
Main Authors: Anbu, P., Gopinath, S.C.B., Hilda, A., Lakshmipriya, T., Annadurai, G.
Format: Article
Language:English
Subjects:
Animal productions
Animals
Biological and medical sciences
Box–Behnken
Catalysis
Culture Media
Fundamental and applied biological sciences. Psychology
Fungi - enzymology
Hydrogen-Ion Concentration
Keratin
Keratinase
Optimization
Peptide Hydrolases - biosynthesis
Poultry - microbiology
Scopulariopsis brevicaulis
Temperature
Terrestrial animal productions
Vertebrates
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Summary:A Scopulariopsis brevicaulis poultry farm isolate was chosen to study factors influencing keratinase production. The parameters were optimized by factorial design. The highest enzyme production by this fungus was obtained at pH 7.5, a temperature of 30 °C and a growth period of 5 weeks. The production of the enzyme was enhanced when the culture medium was supplemented with glucose (1%), sodium nitrate (2%), feather (1.5%) and CaCl 2 (1 mM). According to the responses from the experimental design, the effects of each variable were calculated, and the interactions between them were determined. The experimental values were found to be in accordance with the predicted values, the correlation coefficient is 0.9978.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2006.05.047