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Sensitive high-performance liquid chromatography–tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine
A method to determine total and free mycophenolic acid (MPA) and its metabolites, the phenolic (MPAG) and acyl (AcMPAG) glucuronides, using HPLC and mass spectrometry was developed. Mean recoveries in plasma and urine samples were >85%, and the lower limits of quantification for MPA, MPAG and AcM...
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Published in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-10, Vol.858 (1), p.159-167 |
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container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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creator | Benoit-Biancamano, Marie-Odile Caron, Patrick Lévesque, Éric Delage, Robert Couture, Félix Guillemette, Chantal |
description | A method to determine total and free mycophenolic acid (MPA) and its metabolites, the phenolic (MPAG) and acyl (AcMPAG) glucuronides, using HPLC and mass spectrometry was developed. Mean recoveries in plasma and urine samples were >85%, and the lower limits of quantification for MPA, MPAG and AcMPAG were 0.05, 0.05 and 0.01
mg/L, respectively. For plasma, the assay was linear over 0.05–50
mg/L for MPA and MPAG, and from 0.01 to 10
mg/L for AcMPAG. A validation study demonstrated good inter- and intra-day precision (CV
≤
11%) and accuracy (bias
≤
16%) and satisfactory specificity and stability. Pharmacokinetic parameters were assessed in plasma and urine from healthy volunteers after an oral dose of mycophenolate mofetil. |
doi_str_mv | 10.1016/j.jchromb.2007.08.023 |
format | article |
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mg/L, respectively. For plasma, the assay was linear over 0.05–50
mg/L for MPA and MPAG, and from 0.01 to 10
mg/L for AcMPAG. A validation study demonstrated good inter- and intra-day precision (CV
≤
11%) and accuracy (bias
≤
16%) and satisfactory specificity and stability. Pharmacokinetic parameters were assessed in plasma and urine from healthy volunteers after an oral dose of mycophenolate mofetil.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2007.08.023</identifier><identifier>PMID: 17827076</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>AcMPAG ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Glucuronide ; Glucuronides - blood ; Glucuronides - metabolism ; Glucuronides - urine ; HPLC ; Humans ; Mass spectrometry ; Medical sciences ; MPAG ; Mycophenolic acid ; Mycophenolic Acid - blood ; Mycophenolic Acid - metabolism ; Mycophenolic Acid - urine ; Pharmacology. Drug treatments ; Reproducibility of Results ; Solid Phase Extraction ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-10, Vol.858 (1), p.159-167</ispartof><rights>2007</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-217137c4a9f3984078a8f336f71eb573308f622f630c9ed4569a4d1acd48f7953</citedby><cites>FETCH-LOGICAL-c488t-217137c4a9f3984078a8f336f71eb573308f622f630c9ed4569a4d1acd48f7953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19154181$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17827076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Benoit-Biancamano, Marie-Odile</creatorcontrib><creatorcontrib>Caron, Patrick</creatorcontrib><creatorcontrib>Lévesque, Éric</creatorcontrib><creatorcontrib>Delage, Robert</creatorcontrib><creatorcontrib>Couture, Félix</creatorcontrib><creatorcontrib>Guillemette, Chantal</creatorcontrib><title>Sensitive high-performance liquid chromatography–tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A method to determine total and free mycophenolic acid (MPA) and its metabolites, the phenolic (MPAG) and acyl (AcMPAG) glucuronides, using HPLC and mass spectrometry was developed. Mean recoveries in plasma and urine samples were >85%, and the lower limits of quantification for MPA, MPAG and AcMPAG were 0.05, 0.05 and 0.01
mg/L, respectively. For plasma, the assay was linear over 0.05–50
mg/L for MPA and MPAG, and from 0.01 to 10
mg/L for AcMPAG. A validation study demonstrated good inter- and intra-day precision (CV
≤
11%) and accuracy (bias
≤
16%) and satisfactory specificity and stability. Pharmacokinetic parameters were assessed in plasma and urine from healthy volunteers after an oral dose of mycophenolate mofetil.</description><subject>AcMPAG</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Glucuronide</subject><subject>Glucuronides - blood</subject><subject>Glucuronides - metabolism</subject><subject>Glucuronides - urine</subject><subject>HPLC</subject><subject>Humans</subject><subject>Mass spectrometry</subject><subject>Medical sciences</subject><subject>MPAG</subject><subject>Mycophenolic acid</subject><subject>Mycophenolic Acid - blood</subject><subject>Mycophenolic Acid - metabolism</subject><subject>Mycophenolic Acid - urine</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Solid Phase Extraction</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkU2O1DAQRiMEYoaBI4C8gV2CHSexs0JoxJ80EgtAYmdVO-WOW0mctp2RsuMOHIfbcBLc3ZFmyca27Fdflfyy7CWjBaOseXsoDrr3btwVJaWioLKgJX-UXTMpeM5F8_NxOteC5um6vMqehXCglAkq-NPsiglZpmNznf35hlOw0d4j6e2-z2f0xvkRJo1ksMfFduTcBqLbe5j79e-v3xGmDkcyQggkzKhjesfoV5LW3nUkBZDjAlO0Ec7JMMGwBhuIM2RctZt7nNxgNQGd8lMasTGQ_bDoxbvJdnhKgl1CIgZiJ9IvaSIyDxBGOPOLtxM-z54YGAK-2Pab7MfHD99vP-d3Xz99uX1_l-tKypiXTDAudAWt4a2sqJAgDeeNEQx3teCcStOUpWk41S12Vd20UHUMdFdJI9qa32RvLrmzd8cFQ1SjDRqHASZ0S1CN5HXLZJPA-gJq70LwaNTs7Qh-VYyqkzV1UJs1dbKmqFRJT6p7tTVYdiN2D1WbpgS83gAIGgbjkx8bHriW1RWTLHHvLhym77i36FXQFpPLzvrkSXXO_meUf3R3v0o</recordid><startdate>20071015</startdate><enddate>20071015</enddate><creator>Benoit-Biancamano, Marie-Odile</creator><creator>Caron, Patrick</creator><creator>Lévesque, Éric</creator><creator>Delage, Robert</creator><creator>Couture, Félix</creator><creator>Guillemette, Chantal</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071015</creationdate><title>Sensitive high-performance liquid chromatography–tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine</title><author>Benoit-Biancamano, Marie-Odile ; Caron, Patrick ; Lévesque, Éric ; Delage, Robert ; Couture, Félix ; Guillemette, Chantal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-217137c4a9f3984078a8f336f71eb573308f622f630c9ed4569a4d1acd48f7953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>AcMPAG</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Glucuronide</topic><topic>Glucuronides - blood</topic><topic>Glucuronides - metabolism</topic><topic>Glucuronides - urine</topic><topic>HPLC</topic><topic>Humans</topic><topic>Mass spectrometry</topic><topic>Medical sciences</topic><topic>MPAG</topic><topic>Mycophenolic acid</topic><topic>Mycophenolic Acid - blood</topic><topic>Mycophenolic Acid - metabolism</topic><topic>Mycophenolic Acid - urine</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Solid Phase Extraction</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Benoit-Biancamano, Marie-Odile</creatorcontrib><creatorcontrib>Caron, Patrick</creatorcontrib><creatorcontrib>Lévesque, Éric</creatorcontrib><creatorcontrib>Delage, Robert</creatorcontrib><creatorcontrib>Couture, Félix</creatorcontrib><creatorcontrib>Guillemette, Chantal</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. 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B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2007-10-15</date><risdate>2007</risdate><volume>858</volume><issue>1</issue><spage>159</spage><epage>167</epage><pages>159-167</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A method to determine total and free mycophenolic acid (MPA) and its metabolites, the phenolic (MPAG) and acyl (AcMPAG) glucuronides, using HPLC and mass spectrometry was developed. Mean recoveries in plasma and urine samples were >85%, and the lower limits of quantification for MPA, MPAG and AcMPAG were 0.05, 0.05 and 0.01
mg/L, respectively. For plasma, the assay was linear over 0.05–50
mg/L for MPA and MPAG, and from 0.01 to 10
mg/L for AcMPAG. A validation study demonstrated good inter- and intra-day precision (CV
≤
11%) and accuracy (bias
≤
16%) and satisfactory specificity and stability. Pharmacokinetic parameters were assessed in plasma and urine from healthy volunteers after an oral dose of mycophenolate mofetil.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17827076</pmid><doi>10.1016/j.jchromb.2007.08.023</doi><tpages>9</tpages></addata></record> |
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subjects | AcMPAG Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology General pharmacology Glucuronide Glucuronides - blood Glucuronides - metabolism Glucuronides - urine HPLC Humans Mass spectrometry Medical sciences MPAG Mycophenolic acid Mycophenolic Acid - blood Mycophenolic Acid - metabolism Mycophenolic Acid - urine Pharmacology. Drug treatments Reproducibility of Results Solid Phase Extraction Tandem Mass Spectrometry - methods |
title | Sensitive high-performance liquid chromatography–tandem mass spectrometry method for quantitative analysis of mycophenolic acid and its glucuronide metabolites in human plasma and urine |
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