Effect of nitroxyl on the hamster retinal nitridergic pathway

• Published in: Neurochemistry international 2007-11, Vol.51 (6), p.424-432
• Main Authors: Sáenz, Daniel A., Bari, Sara E., Salido, Ezequiel, Chianelli, Mónica, Rosenstein, Ruth E.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Arginine - metabolism; Biological and medical sciences; Cricetinae; Cyclic GMP - metabolism; Dose-Response Relationship, Drug; Eye and associated structures. Visual pathways and centers. Vision; Fundamental and applied biological sciences. Psychology; Glutathione - drug effects; Glutathione - metabolism; Lipid Peroxidation - drug effects; Lipid Peroxidation - physiology; Male; Mesocricetus; Nitrergic Neurons - metabolism; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide - pharmacology; Nitric Oxide Synthase - drug effects; Nitric Oxide Synthase - metabolism; Nitrites - pharmacology; Nitrogen Oxides - metabolism; Nitrogen Oxides - pharmacology; Nitroxyl; Oxidative Stress - drug effects; Oxidative Stress - physiology; Quaternary Ammonium Compounds - pharmacology; Retina; Retina - drug effects; Retina - metabolism; S-Nitrosothiols - metabolism; Signal Transduction - drug effects; Signal Transduction - physiology; Vertebrates: nervous system and sense organs; Visual Pathways - drug effects; Visual Pathways - metabolism
• ISSN: 0197-0186; 1872-9754
• DOI: 10.1016/j.neuint.2007.04.015

There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO −), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli's salt) and diethylammonium ( Z)-1-( N, N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli's salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli's salt (but not DEA/NO) significantly decreased l-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli's salt affected this parameter with a threshold concentration of 200 μM. Although Angeli's salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing S-nitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response.