HIV-1 Vif protein blocks the cytidine deaminase activity of B-cell specific AID in E. coli by a similar mechanism of action

• Published in: Molecular Immunology 2007, Vol.44 (4), p.583-590
• Main Authors: Santa-Marta, Mariana, Aires da Silva, Frederico, Fonseca, Ana Margarida, Rato, Sylvie, Goncalves, Joao
• Format: Article
• Language: English
• Subjects: Activation-induced deaminase; AID; Amino Acid Sequence; Amino Acid Substitution; Animals; APOBEC-3G Deaminase; APOBEC3G; B-Lymphocytes - enzymology; B-Lymphocytes - virology; Cytidine Deaminase - antagonists & inhibitors; Cytidine Deaminase - genetics; Cytidine deaminases; Escherichia coli; Gene Products, vif - genetics; Gene Products, vif - metabolism; Gene Rearrangement, B-Lymphocyte; HIV-1; Human immunodeficiency virus 1; Humans; Mice; Molecular Sequence Data; Mutation; Nucleoside Deaminases - antagonists & inhibitors; Nucleoside Deaminases - genetics; Repressor Proteins - antagonists & inhibitors; Repressor Proteins - genetics; Vif; vif Gene Products, Human Immunodeficiency Virus
• ISSN: 0161-5890; 1872-9142; 1365-2567
• DOI: 10.1016/j.molimm.2006.02.005

HIV-1 Vif protein protects viral replication in non-permissive cells by inducing degradation of APOBEC3G via ubiquitination and proteasomal pathway, although new studies indicate a putative role in Vif's direct inhibition of APOBEC3G. APOBEC3G is member of a homologous family of proteins with cytidine deaminase activity expressed with characteristic tissue specificity, that in humans consist of APOBEC1, APOBEC2, APOBEC3A-H, APOBEC4 and the activation-induced deaminase (AID), a B lymphoid protein necessary for somatic hypermutation, gene conversion and class switch recombination. In this work we show that Vif can counteract AID's activity in E. coli in absence of specific eukaryotic co-factors necessary for AID induced somatic hypermutation, gene conversion and to stimulate class switch recombination in B-cells. We show that AID inhibition is mediated by a direct protein–protein interaction via unique amino acid D118 an homologous mutant responsible for the species-specific restriction of HIV-1 Vif protein existent for APOBEC3G. These results raise the hypothesis that Vif related proteins can act as a broad inhibitor of deaminase activity. Moreover as AID and Vif evolved in different cellular environments, these results may indicate that Vif related proteins might mimic cellular factors that interact with a structural conserved domain of cytidine deaminases during evolution.