Relations between relative mRNA abundance and developmental competence of ovine oocytes

The present study was conducted to investigate the relation between in vitro developmental competence and the expression of a panel of developmentally important genes in germinal vesicle (GV) stage oocytes. One‐month‐old prepubertal and adult sheep oocytes were used as models of low and high quality...

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Bibliographic Details
Published in:Molecular reproduction and development 2007-02, Vol.74 (2), p.249-257
Main Authors: Leoni, G.G., Bebbere, D., Succu, S., Berlinguer, F., Mossa, F., Galioto, M., Bogliolo, L., Ledda, S., Naitana, S.
Format: Article
Language:English
Subjects:
Aging
Animals
Base Sequence
Biological and medical sciences
DNA Primers
Enzymes - genetics
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Gene expression
kinetic of development
Maternal-Fetal Exchange
Molecular and cellular biology
Molecular genetics
oocyte quality
Oocytes - physiology
Ovary - cytology
Ovary - physiology
Pregnancy
RNA, Messenger - genetics
Sexual Maturation
Sheep - growth & development
Transcription, Genetic
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Summary:The present study was conducted to investigate the relation between in vitro developmental competence and the expression of a panel of developmentally important genes in germinal vesicle (GV) stage oocytes. One‐month‐old prepubertal and adult sheep oocytes were used as models of low and high quality gametes, respectively. Cumulus‐oocyte complexes (COCs) derived from lambs and ewes were in vitro matured and fertilized, and their cleavage rate at 22, 26, and 32 hr post fertilization and the blastocyst yield were observed to assess their developmental potential. In parallel, the relative abundance (RA) of 11 genes was analyzed by semi‐quantitative Reverse Transcription Polymerase Chain Reaction (RT‐PCR) assay in the two groups of oocytes. We observed similar maturation and fertilization rates in the two groups, but a significant lower rate of cleaved prepubertal oocytes (P 
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20442