Beneficial effects of prolactin and laminin on human pancreatic islet-cell cultures

The problem of pancreas donor shortage could be addressed through in vitro islet-cell proliferation prior to transplantation into diabetic patients. Therefore, we set out to evaluate the effects of prolactin (rhPRL) and laminin on primary cultures of human pancreatic islets. Our results showed that...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular endocrinology 2007-01, Vol.263 (1), p.120-133
Main Authors: Labriola, Leticia, Montor, Wagner R., Krogh, Karin, Lojudice, Fernando H., Genzini, Tércio, Goldberg, Anna C., Eliaschewitz, Freddy G., Sogayar, Mari C.
Format: Article
Language:English
Subjects:
Adult
Aged
Beta-cell proliferation
Blotting, Western
Cell Proliferation - drug effects
Cells, Cultured - drug effects
Diabetes
Enzyme Activation - drug effects
Female
Fluorescent Antibody Technique
Glucose - pharmacology
Human Pancreatic islets
Humans
Immunoprecipitation
Insulin
Insulin - metabolism
Insulin Secretion
Islets of Langerhans - cytology
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Janus Kinase 2 - metabolism
Laminin
Laminin - pharmacology
Male
Middle Aged
Prolactin
Prolactin - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
STAT
STAT Transcription Factors - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The problem of pancreas donor shortage could be addressed through in vitro islet-cell proliferation prior to transplantation into diabetic patients. Therefore, we set out to evaluate the effects of prolactin (rhPRL) and laminin on primary cultures of human pancreatic islets. Our results showed that rhPRL induced an increase in islet-cell number and in cumulative insulin secretion ( p < 0.01). However, glucose-induced insulin secretion was enhanced only in the presence of both laminin and rhPRL. In addition, we describe, for the first time in human islets, the PRL-induced activation of JAK2, and signal transducer and activator of transcription (STAT) 1, 3 and 5. Our results demonstrate a significant beneficial effect of rhPRL and laminin on human islets and support widely held notion that the closer physiological stimuli and environment of beta cells are mimicked, the better are the results in cell proliferation and secretory function, both essential for successful islet transplantation.
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2006.09.011