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Enhancement of transglutaminase activity and polyamine depletion in B16-F10 melanoma cells by flavonoids naringenin and hesperitin correlate to reduction of the in vivo metastatic potential
The in vitro and in vivo effects of two flavonons, naringenin (NG) and hesperitin (HP) on the proliferation rate of highly metastatic murine B16-F10 melanoma cell were investigated. NG or HP treatment of melanoma cells produced a remarkable reduction of cell proliferation, paralleled with both the l...
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Published in: | Amino acids 2007, Vol.32 (1), p.95-100 |
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description | The in vitro and in vivo effects of two flavonons, naringenin (NG) and hesperitin (HP) on the proliferation rate of highly metastatic murine B16-F10 melanoma cell were investigated. NG or HP treatment of melanoma cells produced a remarkable reduction of cell proliferation, paralleled with both the lowering of the intracellular levels of polyamine, spermidine and spermine and the enhancement of transglutaminase (TGase, EC 2.3.2.13) activity. Orally administered NG or HP in C57BL6/N mice inoculated with B16-F10 cells affected the pulmonary invasion of melanoma cells in an in vivo metastatic assay. The number of lung metastases detected by a computerized image analyzer was reduced, compared to untreated animals, by about 69% in NG-treated mice and by about 36% in HP-treated mice. Survival studies showed that 50% of the NG-treated animals died 38 ± 3.1 days after tumor cell injection (control group: 18 ± 1.5 days) and HP-treated mice died 27 ± 2.3 days after cell inoculation. Taken together, these findings provide further evidences for the potential anticancer properties of dietary flavonoids as chemopreventive agents against malignant melanoma. |
doi_str_mv | 10.1007/s00726-006-0304-3 |
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NG or HP treatment of melanoma cells produced a remarkable reduction of cell proliferation, paralleled with both the lowering of the intracellular levels of polyamine, spermidine and spermine and the enhancement of transglutaminase (TGase, EC 2.3.2.13) activity. Orally administered NG or HP in C57BL6/N mice inoculated with B16-F10 cells affected the pulmonary invasion of melanoma cells in an in vivo metastatic assay. The number of lung metastases detected by a computerized image analyzer was reduced, compared to untreated animals, by about 69% in NG-treated mice and by about 36% in HP-treated mice. Survival studies showed that 50% of the NG-treated animals died 38 ± 3.1 days after tumor cell injection (control group: 18 ± 1.5 days) and HP-treated mice died 27 ± 2.3 days after cell inoculation. Taken together, these findings provide further evidences for the potential anticancer properties of dietary flavonoids as chemopreventive agents against malignant melanoma.</description><identifier>ISSN: 0939-4451</identifier><identifier>EISSN: 1438-2199</identifier><identifier>DOI: 10.1007/s00726-006-0304-3</identifier><identifier>PMID: 16699821</identifier><language>eng</language><publisher>Austria: Vienna : Springer-Verlag</publisher><subject>Animals ; Antineoplastic Agents - pharmacology ; Antineoplastic Agents - therapeutic use ; Biocompatibility ; Biomedical materials ; Cell Proliferation - drug effects ; Enzyme Activation - drug effects ; Estrogen Antagonists - pharmacology ; Estrogen Antagonists - therapeutic use ; Flavanones - pharmacology ; Flavanones - therapeutic use ; flavonoids ; In vivo testing ; In vivo tests ; melanoma ; Melanoma - enzymology ; Melanoma - prevention & control ; metastasis ; Mice ; Neoplasm Metastasis ; Neoplasm Proteins - agonists ; Neoplasm Proteins - metabolism ; Polyamines ; protein-glutamine gamma-glutamyltransferase ; Reduction ; Skin cancer ; Spermidine - metabolism ; Spermine - metabolism ; Surgical implants ; Transglutaminases - metabolism</subject><ispartof>Amino acids, 2007, Vol.32 (1), p.95-100</ispartof><rights>Springer-Verlag 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-8e26c7bec831dd1ea8ccb9cbec019e403c11ca05f2e8abbe77e5d8db2d8eb2983</citedby><cites>FETCH-LOGICAL-c384t-8e26c7bec831dd1ea8ccb9cbec019e403c11ca05f2e8abbe77e5d8db2d8eb2983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16699821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lentini, A</creatorcontrib><creatorcontrib>Forni, C</creatorcontrib><creatorcontrib>Provenzano, B</creatorcontrib><creatorcontrib>Beninati, S</creatorcontrib><title>Enhancement of transglutaminase activity and polyamine depletion in B16-F10 melanoma cells by flavonoids naringenin and hesperitin correlate to reduction of the in vivo metastatic potential</title><title>Amino acids</title><addtitle>Amino Acids</addtitle><description>The in vitro and in vivo effects of two flavonons, naringenin (NG) and hesperitin (HP) on the proliferation rate of highly metastatic murine B16-F10 melanoma cell were investigated. NG or HP treatment of melanoma cells produced a remarkable reduction of cell proliferation, paralleled with both the lowering of the intracellular levels of polyamine, spermidine and spermine and the enhancement of transglutaminase (TGase, EC 2.3.2.13) activity. Orally administered NG or HP in C57BL6/N mice inoculated with B16-F10 cells affected the pulmonary invasion of melanoma cells in an in vivo metastatic assay. The number of lung metastases detected by a computerized image analyzer was reduced, compared to untreated animals, by about 69% in NG-treated mice and by about 36% in HP-treated mice. Survival studies showed that 50% of the NG-treated animals died 38 ± 3.1 days after tumor cell injection (control group: 18 ± 1.5 days) and HP-treated mice died 27 ± 2.3 days after cell inoculation. Taken together, these findings provide further evidences for the potential anticancer properties of dietary flavonoids as chemopreventive agents against malignant melanoma.</description><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Cell Proliferation - drug effects</subject><subject>Enzyme Activation - drug effects</subject><subject>Estrogen Antagonists - pharmacology</subject><subject>Estrogen Antagonists - therapeutic use</subject><subject>Flavanones - pharmacology</subject><subject>Flavanones - therapeutic use</subject><subject>flavonoids</subject><subject>In vivo testing</subject><subject>In vivo tests</subject><subject>melanoma</subject><subject>Melanoma - enzymology</subject><subject>Melanoma - prevention & control</subject><subject>metastasis</subject><subject>Mice</subject><subject>Neoplasm Metastasis</subject><subject>Neoplasm Proteins - agonists</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Polyamines</subject><subject>protein-glutamine gamma-glutamyltransferase</subject><subject>Reduction</subject><subject>Skin cancer</subject><subject>Spermidine - metabolism</subject><subject>Spermine - metabolism</subject><subject>Surgical implants</subject><subject>Transglutaminases - metabolism</subject><issn>0939-4451</issn><issn>1438-2199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9ktGK1DAUhoso7rj6AN5oQBBvqjlNmyaXuuyuwoIXutflND2dydImY5IOzMP5bqY7A4IXXiQhyXf-_If8RfEa-EfgvP0U81TJkvM8BK9L8aTYQC1UWYHWT4sN10KXdd3ARfEixgfOoVIgnxcXIKXWqoJN8fva7dAZmskl5keWArq4nZaEs3UYiaFJ9mDTkaEb2N5Px_WC2ED7iZL1jlnHvoAsb4CzmSZ0fkZmaJoi649snPDgnbdDZA6DdVtymV-ldhT3FGzKW-NDyJWJWPIs0LCYR-HVzY5W_YM9-CyeMCZM1mQbKdu1OL0sno04RXp1Xi-L-5vrn1dfy7vvt9-uPt-VRqg6lYoqadqejBIwDECojOm1yQccNNVcGACDvBkrUtj31LbUDGroq0FRX2klLov3J9198L8WiqmbbVybREd-iZ1UQirFRQY__BeE_KCUolY6o-_-QR_8ElxuI1O6kaoSqskUnCgTfIyBxm4f7IzhmKFuDUF3CkGXQ9CtIehWE2_Oyks_0_C34vzrGXh7Akb0HW6Djd39j4qD4ADZX1uLPzbRuxY</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Lentini, A</creator><creator>Forni, C</creator><creator>Provenzano, B</creator><creator>Beninati, S</creator><general>Vienna : Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Enhancement of transglutaminase activity and polyamine depletion in B16-F10 melanoma cells by flavonoids naringenin and hesperitin correlate to reduction of the in vivo metastatic potential</title><author>Lentini, A ; Forni, C ; Provenzano, B ; Beninati, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-8e26c7bec831dd1ea8ccb9cbec019e403c11ca05f2e8abbe77e5d8db2d8eb2983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Cell Proliferation - drug effects</topic><topic>Enzyme Activation - drug effects</topic><topic>Estrogen Antagonists - pharmacology</topic><topic>Estrogen Antagonists - therapeutic use</topic><topic>Flavanones - pharmacology</topic><topic>Flavanones - therapeutic use</topic><topic>flavonoids</topic><topic>In vivo testing</topic><topic>In vivo tests</topic><topic>melanoma</topic><topic>Melanoma - enzymology</topic><topic>Melanoma - prevention & control</topic><topic>metastasis</topic><topic>Mice</topic><topic>Neoplasm Metastasis</topic><topic>Neoplasm Proteins - agonists</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Polyamines</topic><topic>protein-glutamine gamma-glutamyltransferase</topic><topic>Reduction</topic><topic>Skin cancer</topic><topic>Spermidine - metabolism</topic><topic>Spermine - metabolism</topic><topic>Surgical implants</topic><topic>Transglutaminases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lentini, A</creatorcontrib><creatorcontrib>Forni, C</creatorcontrib><creatorcontrib>Provenzano, B</creatorcontrib><creatorcontrib>Beninati, S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Amino acids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lentini, A</au><au>Forni, C</au><au>Provenzano, B</au><au>Beninati, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of transglutaminase activity and polyamine depletion in B16-F10 melanoma cells by flavonoids naringenin and hesperitin correlate to reduction of the in vivo metastatic potential</atitle><jtitle>Amino acids</jtitle><addtitle>Amino Acids</addtitle><date>2007</date><risdate>2007</risdate><volume>32</volume><issue>1</issue><spage>95</spage><epage>100</epage><pages>95-100</pages><issn>0939-4451</issn><eissn>1438-2199</eissn><abstract>The in vitro and in vivo effects of two flavonons, naringenin (NG) and hesperitin (HP) on the proliferation rate of highly metastatic murine B16-F10 melanoma cell were investigated. NG or HP treatment of melanoma cells produced a remarkable reduction of cell proliferation, paralleled with both the lowering of the intracellular levels of polyamine, spermidine and spermine and the enhancement of transglutaminase (TGase, EC 2.3.2.13) activity. Orally administered NG or HP in C57BL6/N mice inoculated with B16-F10 cells affected the pulmonary invasion of melanoma cells in an in vivo metastatic assay. The number of lung metastases detected by a computerized image analyzer was reduced, compared to untreated animals, by about 69% in NG-treated mice and by about 36% in HP-treated mice. Survival studies showed that 50% of the NG-treated animals died 38 ± 3.1 days after tumor cell injection (control group: 18 ± 1.5 days) and HP-treated mice died 27 ± 2.3 days after cell inoculation. Taken together, these findings provide further evidences for the potential anticancer properties of dietary flavonoids as chemopreventive agents against malignant melanoma.</abstract><cop>Austria</cop><pub>Vienna : Springer-Verlag</pub><pmid>16699821</pmid><doi>10.1007/s00726-006-0304-3</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Antineoplastic Agents - pharmacology Antineoplastic Agents - therapeutic use Biocompatibility Biomedical materials Cell Proliferation - drug effects Enzyme Activation - drug effects Estrogen Antagonists - pharmacology Estrogen Antagonists - therapeutic use Flavanones - pharmacology Flavanones - therapeutic use flavonoids In vivo testing In vivo tests melanoma Melanoma - enzymology Melanoma - prevention & control metastasis Mice Neoplasm Metastasis Neoplasm Proteins - agonists Neoplasm Proteins - metabolism Polyamines protein-glutamine gamma-glutamyltransferase Reduction Skin cancer Spermidine - metabolism Spermine - metabolism Surgical implants Transglutaminases - metabolism |
title | Enhancement of transglutaminase activity and polyamine depletion in B16-F10 melanoma cells by flavonoids naringenin and hesperitin correlate to reduction of the in vivo metastatic potential |
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