Effects of probe binding mutations in an assay designed to detect parvovirus B19: Implications for the quantitation of different virus genotypes

Quantitative real-time PCR is being widely used in the identification of plasma donations that contain high levels of parvovirus B19, to ensure their exclusion from start pools used in the manufacture of plasma derived medicinal products. In this study, the primers and probe of one such published as...

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Bibliographic Details
Published in:Journal of virological methods 2007, Vol.139 (1), p.97-99
Main Authors: Baylis, Sally A., Fryer, Jacqueline F., Grabarczyk, Piotr
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genotype
Microbiology
Mutation
Parvovirus B19
Parvovirus B19, Human - classification
Parvovirus B19, Human - genetics
Parvovirus B19, Human - isolation & purification
Plasma
Polymerase Chain Reaction - methods
Real-time PCR
Techniques used in virology
Variants
Virology
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Summary:Quantitative real-time PCR is being widely used in the identification of plasma donations that contain high levels of parvovirus B19, to ensure their exclusion from start pools used in the manufacture of plasma derived medicinal products. In this study, the primers and probe of one such published assay, are examined for their ability to quantify different genotypes of parvovirus B19. Under standard assay conditions, there is a failure to detect and quantify one genotype 3 subtype. Alterations in assay conditions can restore quantitation of this subtype.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2006.09.002