Complete sequence and analysis of the ovine herpesvirus 2 genome

Ovine herpesvirus 2 (OvHV-2) is endemic in sheep populations worldwide and causes malignant catarrhal fever (MCF), a lymphoproliferative disease, in cattle, bison and deer. OvHV-2 has been placed in the gammaherpesvirus subfamily and is related closely to Alcelaphine herpesvirus 1 (AlHV-1). Here, th...

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Bibliographic Details
Published in:Journal of general virology 2007, Vol.88 (1), p.28-39
Main Authors: Hart, J, Ackermann, M, Jayawardane, G, Russell, G, Haig, D.M, Reid, H, Stewart, J.P
Format: Article
Language:English
Subjects:
Alcelaphine herpesvirus 1
Animals
Biological and medical sciences
Bison
Cattle
Cell Line
DNA
DNA, Viral - analysis
exons
Fundamental and applied biological sciences. Psychology
Gammaherpesvirus
Genes, Viral - genetics
genome
Genome, Viral
Microbiology
Miscellaneous
molecular cloning
Molecular Sequence Data
nucleotide sequences
Open Reading Frames
Ovine herpesvirus 2
Rhadinovirus - genetics
Sequence Analysis, DNA
Sheep - virology
terminal repeat sequences
Virology
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Summary:Ovine herpesvirus 2 (OvHV-2) is endemic in sheep populations worldwide and causes malignant catarrhal fever (MCF), a lymphoproliferative disease, in cattle, bison and deer. OvHV-2 has been placed in the gammaherpesvirus subfamily and is related closely to Alcelaphine herpesvirus 1 (AlHV-1). Here, the cloning, sequencing and analysis of the complete OvHV-2 genome derived from a lymphoblastoid cell line from an affected cow (BJ1035) are reported. The unique portion of the genome consists of 130 930 bp, with a mean G+C content of 52 mol%. The unique DNA is flanked by multiple copies of terminal repeat elements 4205 bp in length, with a mean G+C content of 72 mol%. Analysis revealed 73 open reading frames (ORFs), the majority (62) of which showed homology to other gammaherpesvirus genes. A further subset of nine ORFs is shared with only the related AlHV-1. Three ORFs are entirely unique to OvHV-2, including a spliced homologue of cellular interleukin-10 that retains the exon structure of the cellular gene. The sequence of OvHV-2 is a critical first step in the study of the pathogenesis and treatment of MCF.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.82284-0