Overproduction of PGE2 in peripheral blood monocytes of gastrointestinal cancer patients with mucins in their bloodstream

Abstract When monocytes from healthy donors were cultured in the presence of sera from patients with gastrointestinal cancer, PGE2 production from the monocytes was elevated. Serum proteins were fractionated on Sepharose 4B and the inducing activity was found in the excluded fractions. By excluding...

Full description

Saved in:
Bibliographic Details
Published in:Cancer letters 2007-01, Vol.245 (1), p.149-155
Main Authors: Yokoigawa, Norio, Takeuchi, Noriko, Toda, Munetoyo, Inoue, Mizue, Kaibori, Masaki, Yanagida, Hidesuke, Inaba, Takaaki, Tanaka, Hironori, Ogura, Tokuhiro, Takada, Hideho, Okumura, Tadayoshi, Kwon, A-Hon, Kamiyama, Yasuo, Nakada, Hiroshi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Binding sites
Blood Proteins - pharmacology
Cells, Cultured
Colorectal cancer
Cyclooxygenase 2 - genetics
Cyclooxygenase Inhibitors - pharmacology
Dinoprostone - biosynthesis
Enzyme-Linked Immunosorbent Assay
Enzymes
Etodolac - pharmacology
Gastrointestinal cancer
Gastrointestinal Neoplasms - blood
Gastrointestinal Neoplasms - enzymology
Gastrointestinal Neoplasms - genetics
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Neoplastic - drug effects
Hematology, Oncology and Palliative Medicine
Humans
Indomethacin - pharmacology
Ligands
Molecular Sequence Data
Monocytes
Monocytes - cytology
Monocytes - drug effects
Monocytes - metabolism
Mucin
Mucins - blood
Mucins - pharmacology
Oligopeptides - pharmacology
Peptides
PGE2
Receptors, Scavenger - chemistry
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Scavenger receptor
Signal transduction
Sulfoglycosphingolipids - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract When monocytes from healthy donors were cultured in the presence of sera from patients with gastrointestinal cancer, PGE2 production from the monocytes was elevated. Serum proteins were fractionated on Sepharose 4B and the inducing activity was found in the excluded fractions. By excluding some mucins from the serum, the inducing activity was reduced effectively. The activity was also reduced by adding binding inhibitors to the scavenger receptor. These results suggest that peripheral blood monocytes in epithelial cancer patients may be continuously stimulated by mucins in the bloodstream through the scavenger receptor, resulting in overproduction of PGE2.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2006.01.004