Analysis of different β-lactams antibiotics in pharmaceutical preparations using micellar electrokinetic capillary chromatography

The potential of micellar electrokinetic capillary chromatography (MEKC) for analyzing nine β-lactams antibiotics (cloxacillin, dicloxacillin, oxacillin, penicillin G, penicillin V, ampicillin, nafcillin, piperacillin, amoxicillin) in different pharmaceutical preparations, have been demonstrated. An...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2007-01, Vol.43 (2), p.746-752
Main Authors: BAILON PEREZ, M. I, CUADROS RODRIGUEZ, L, CRUCES-BLANCO, C
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Anti-Bacterial Agents - analysis
Antibiotics
beta-Lactams - analysis
Biological and medical sciences
Buffers
Calibration
Capillary electrophoresis
Capsules
Chromatography, Micellar Electrokinetic Capillary - methods
Chromatography, Micellar Electrokinetic Capillary - standards
Fundamental and applied biological sciences. Psychology
General pharmacology
Hydrogen-Ion Concentration
Medical sciences
Molecular Structure
Pharmaceutical analysis
Pharmaceutical Preparations - chemistry
Pharmaceutical Preparations - standards
Pharmaceutical Solutions
Pharmacology. Drug treatments
Quality Control
Reproducibility of Results
Spectrophotometry, Ultraviolet - methods
Tablets
Technology, Pharmaceutical - methods
Technology, Pharmaceutical - standards
Temperature
Veterinary Drugs - chemistry
Veterinary Drugs - standards
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Summary:The potential of micellar electrokinetic capillary chromatography (MEKC) for analyzing nine β-lactams antibiotics (cloxacillin, dicloxacillin, oxacillin, penicillin G, penicillin V, ampicillin, nafcillin, piperacillin, amoxicillin) in different pharmaceutical preparations, have been demonstrated. An experimental design strategy has been applied to optimize the main variables: pH and buffer concentration, concentration of the micellar medium, separation voltage and capillary temperature. Borate buffer (26 mM) at pH 8.5 containing 100 mM sodium dodecyl sulphate (SDS) was used as the background electrolyte. The method was validated. Linearity, limit of detection and quantitation and precision were established for each compound. The analysis of some of the β-lactams in Orbenin capsules, Britapen tables and in Veterin–Micipen injectable, all used in human and veterinary medicine, have demonstrated the applicability of these technique for quality control in the pharmaceutical industry.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2006.07.035