Microbial sensors for small molecules: Development of a mevalonate biosensor

We describe a novel biosensor strain for detection and quantification of a small molecule, mevalonate. The biosensor strain is an Escherichia coli mevalonate auxotroph that expresses the green fluorescent protein and reports on the mevalonate concentration in the growth medium through a change in gr...

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Bibliographic Details
Published in:Metabolic engineering 2007, Vol.9 (1), p.30-38
Main Authors: Pfleger, Brian F., Pitera, Douglas J., Newman, Jack D., Martin, Vincent J.J., Keasling, Jay D.
Format: Article
Language:English
Subjects:
Aldose-Ketose Isomerases - physiology
Base Sequence
Biosensing Techniques - methods
Biosensor
DXP Pathway
Escherichia coli
Escherichia coli - metabolism
Gas Chromatography-Mass Spectrometry
High-throughput screening
Isoprenoid
Mevalonate
Mevalonate Pathway
Mevalonic Acid - analysis
Molecular Sequence Data
Multienzyme Complexes - physiology
Oxidoreductases - physiology
Terpenes - metabolism
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Description
Summary:We describe a novel biosensor strain for detection and quantification of a small molecule, mevalonate. The biosensor strain is an Escherichia coli mevalonate auxotroph that expresses the green fluorescent protein and reports on the mevalonate concentration in the growth medium through a change in growth rate. A model describing the growth rate dependence on mevalonate was developed in order to use the biosensor strain for high-throughput screening (HTS) and quantitative measurement of mevalonate in the extracellular environment. In general, this method should be applicable to the quantification of any small molecule for which an auxotroph can be developed and will be useful for HTS of evolved metabolic pathways for which there is no readily available screen or selection.
ISSN:1096-7176
1096-7184
DOI:10.1016/j.ymben.2006.08.002