Antibacterial cathelicidin peptide CAP11 suppresses the anandamide production from lipopolysaccharide-stimulated mononuclear phagocytes

The action of antibacterial cathelicidin peptide CAP11 on the anandamide production from mononuclear phagocytes was examined. Lipopolysaccharide (LPS)-stimulation induced the anandamide production from macrophage-like RAW264.7, accompanied with the enhanced anandamide-synthesizing enzyme activity; h...

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Bibliographic Details
Published in:FEBS letters 2007-01, Vol.581 (1), p.140-144
Main Authors: Murakami, Taisuke, Yomogida, Shin, Someya, Akimasa, Kuwahara-Arai, Kyoko, Tamura, Hiroshi, Nagaoka, Isao
Format: Article
Language:English
Subjects:
4-(N,N-dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole
Amidohydrolases - metabolism
Anandamide
Animals
Anti-Bacterial Agents - pharmacology
Antibacterial peptide
Antimicrobial Cationic Peptides - pharmacology
Arachidonic Acids - biosynthesis
CAP11
Cathelicidin
cationic antibacterial peptide of 11-kDa
Cell Line
DBD-COCL
Endocannabinoids
Enzyme Activation - drug effects
FAAH
fatty acid amide hydrolase
FCS
fetal calf serum
Lipopolysaccharide
Lipopolysaccharide Receptors - metabolism
Lipopolysaccharides - pharmacology
LPS
Macrophages - enzymology
Mice
Mononuclear phagocyte
N-arachidonyl phosphatidylethanolamine hydrolyzing phospholipase D
NAPE-PLD
Polyunsaturated Alkamides
Protein Binding - drug effects
reverse transcription-polymerase chain reaction
RT-PCR
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Summary:The action of antibacterial cathelicidin peptide CAP11 on the anandamide production from mononuclear phagocytes was examined. Lipopolysaccharide (LPS)-stimulation induced the anandamide production from macrophage-like RAW264.7, accompanied with the enhanced anandamide-synthesizing enzyme activity; however, the anandamide-degrading enzyme activity was not changed by LPS-stimulation. Importantly, CAP11 suppressed the LPS-induced anandamide production and the increase of anandamide-synthesizing enzyme activity. Furthermore, CAP11 abrogated the LPS-binding to CD14-positive RAW264.7. These observations indicate that CAP11 inhibits the binding of LPS to CD14-positive mononuclear phagocytes, thereby suppressing the anandamide synthesizing enzyme activity and the anandamide production from the cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.12.017