Visceral and orofacial somatic afferent fiber terminals converge onto the same neuron in paratrigeminal nucleus: An electron microscopic study in rats

• Published in: Autonomic neuroscience 2007-01, Vol.131 (1), p.45-49
• Main Authors: Ma, Wen-Ling, Zhang, Wen-Bin, Xiong, Kang-Hui, Guo, Feng
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Afferent Pathways - physiology; Animals; Biological and medical sciences; Convergent neuron; Degeneration; Fundamental and applied biological sciences. Psychology; Geniculate Ganglion - physiology; Glossopharyngeal nerve; Glossopharyngeal Nerve - physiology; Horseradish peroxidase (HRP); Male; Medical Education; Microscopy, Electron, Transmission - methods; Neurons, Afferent - physiology; Neurons, Afferent - ultrastructure; Paratrigeminal nucleus (Pa5); Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ; Rats; Rats, Sprague-Dawley; Trigeminal nerve; Trigeminal Nuclei - cytology; Vagal nerve; Vagus Nerve - physiology; Vertebrates: nervous system and sense organs; Wallerian Degeneration - pathology; Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate - metabolism
• ISSN: 1566-0702; 1872-7484
• DOI: 10.1016/j.autneu.2006.06.006

Abstract The paratrigeminal nucleus (Pa5) receives visceral sensory inputs through the vagus (X) and glossopharyngeal (IX) nerves and somatic sensory inputs through the trigeminal (V) nerve. In the present study, transganglionic transport of the WGA–HRP and Wallerian degeneration was used to identify whether two kinds of primary afferent fiber terminals converge onto a single neuron in the Pa5 at the utrastructural level. It was found that HRP-labeled and degenerated terminals originating from the IX and/or X nerves and infraorbital nerve formed asymmetrical synapses with unlabeled dendrites in the Pa5. Furthermore, approximately 7% (43/630) HRP-labeled and 31% (43/137) degenerated terminals formed synaptic connections with the same dendritic profiles simultaneously in the dorsal division of the Pa5. These results may provide a neuroanatomical substrate for integration of viscerosomatic sensory inputs associated with visceral and cardiovascular reflexes in the Pa5.