Molecular typing of wine yeast strains Saccharomyces bayanus var. uvarum using microsatellite markers

The Saccharomyces bayanus var . uvarum yeasts are associated with spontaneous fermentation of must. Some strains were shown to be enological yeasts of interest in different winemaking processes. The molecular typing of S. bayanus var . uvarum at the strain level has become significant for wine micro...

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Bibliographic Details
Published in:Systematic and applied microbiology 2007-01, Vol.30 (1), p.75-82
Main Authors: Masneuf-Pomarède, I., Le Jeune, C., Durrens, P., Lollier, M., Aigle, M., Dubourdieu, D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chimera
Electrophoresis - methods
Fundamental and applied biological sciences. Psychology
Genome, Fungal
Hybrid
Karyotyping
Microbiology
Microsatellite
Microsatellite Repeats
Miscellaneous
Molecular typing
Mycological Typing Techniques
Mycology
Saccharomyces - classification
Saccharomyces - genetics
Saccharomyces - isolation & purification
Saccharomyces bayanus
Saccharomyces bayanus var. uvarum
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Vitaceae
Wine - microbiology
Wine yeast
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Summary:The Saccharomyces bayanus var . uvarum yeasts are associated with spontaneous fermentation of must. Some strains were shown to be enological yeasts of interest in different winemaking processes. The molecular typing of S. bayanus var . uvarum at the strain level has become significant for wine microbiologists. Four microsatellite loci were defined from the exploration of genomic DNA sequence of S. bayanus var . uvarum. The 40 strains studied were homozygote for the locus considered. The discriminating capacity of the microsatellite method was found to be equal to that of karyotypes analysis. Links between 37 indigenous strains with the same geographic origin could be established through the analysis of microsatellite patterns. The analysis of microsatellite polymorphism is a reliable method for wine S. bayanus var . uvarum strains and their hybrids with Saccharomyces cerevisiae identification in taxonomic, ecological studies and winemaking applications.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2006.02.006