A novel technique to perform direct intraganglionar injections in rats

The present work describes a simple method for direct drug administration into the dorsal root ganglion (DRG) in anesthetized rats. This technique does not involve surgery, is easy to learn and allows behavioral testing within minutes after the injection. Based on landmarks that target the L5 DRG, a...

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Bibliographic Details
Published in:Journal of neuroscience methods 2007-01, Vol.159 (2), p.236-243
Main Authors: Ferrari, L.F., Cunha, F.Q., Parada, C.A., Ferreira, S.H.
Format: Article
Language:English
Subjects:
Analgesics, Opioid - pharmacology
Anesthesia
Animals
Arginine - pharmacology
Carbon Radioisotopes
Coloring Agents - pharmacology
Dinoprostone - pharmacology
Dorsal root ganglion
DRG injection
Ganglia, Spinal - drug effects
Glyburide - pharmacology
Hindlimb
Hypoglycemic Agents - pharmacology
Intraganglionar injection
Intrathecal injection
Male
Microinjections - instrumentation
Microinjections - methods
Morphine - pharmacology
Needles
Nociceptors - drug effects
Rats
Rats, Wistar
Reflex - drug effects
Reflex - physiology
Trypan Blue - pharmacology
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Summary:The present work describes a simple method for direct drug administration into the dorsal root ganglion (DRG) in anesthetized rats. This technique does not involve surgery, is easy to learn and allows behavioral testing within minutes after the injection. Based on landmarks that target the L5 DRG, an orifice was created with a guide needle through which a specially designed needle was inserted for solution injection. Its introduction into the ganglia was ensured by the triggering of an ipsilateral hindpaw reflex. The precision of the technique was checked by injections of the biological dye Pontamine Sky Blue (PSB) or C 14-labeled arginine. There was no leakage of the dye to the surrounding tissues after a single 4 μl or three successive 2.5 μl injections (at 30-min intervals). Moreover, identical effects were observed with prostaglandin E 2 (PGE 2), morphine or glibenclamide injected intraplantarly or in the DRG, thus confirming the precision of the method and suggesting that the ganglion cells and peripheral nociceptors may display similar receptor population.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2006.07.025