Regulation of avilamycin biosynthesis in Streptomyces viridochromogenes: effects of glucose, ammonium ion, and inorganic phosphate

• Published in: Applied microbiology and biotechnology 2007, Vol.73 (5), p.1031-1038
• Main Authors: Zhu, Chuan-he, Lu, Fu-ping, He, Ya-nan, Han, Zhen-lin, Du, Lian-xiang
• Format: Article
• Language: English
• Subjects: 3-O-Methylglucose - pharmacology; Ammonium; ATP; Avilamycin; Biological and medical sciences; Biosynthesis; Biotechnology; Culture Media - chemistry; Dehydrogenase; Dehydrogenases; Deoxyglucose - pharmacology; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - pharmacology; Ions; Oligosaccharides - biosynthesis; Phosphates; Phosphates - pharmacology; Phosphoric Monoester Hydrolases - antagonists & inhibitors; Quaternary Ammonium Compounds - pharmacology; Regulation; Streptomyces - metabolism; Streptomyces viridochromogenes; Streptomyces viridochromogenes AS4.126; Valine Dehydrogenase (NADP+) - antagonists & inhibitors
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-006-0572-6

Effects of glucose, ammonium ions and phosphate on avilamycin biosynthesis in Streptomyces viridochromogenes AS4.126 were investigated. Twenty grams per liter of glucose, 10 mmol/L ammonium ions, and 10 mmol/L phosphate in the basal medium stimulated avilamycin biosynthesis. When the concentrations of glucose, ammonium ions, and phosphate in the basal medium exceeded 20 g/L, 10 mmol/L, and 10 mmol/L, respectively, avilamycin biosynthesis greatly decreased. When 20 g/L glucose was added at 32 h, avilamycin yield decreased by 70.2%. Avilamycin biosynthesis hardly continued when 2-deoxy-glucose was added into the basal medium at 32 h. There was little influence on avilamycin biosynthesis with the addition of the 3-methyl-glucose (20 g/L) at 32 h. In the presence of excess (NH₄)₂SO₄ (20 mmol/L), the activities of valine dehydrogenase and glucose-6-phosphate dehydrogenase were depressed 47.7 and 58.3%, respectively, of that of the control at 48 h. The activity of succinate dehydrogenase increased 49.5% compared to the control at 48 h. The intracellular adenosine triphosphate level and 6-phosphate glucose content of S. viridochromogenes were 128 and 129%, respectively, of that of the control at 48 h, with the addition of the 40 mmol/L of KH₂PO₄. As a result, high concentrations of glucose, ammonium ions, and inorganic phosphate all led to the absence of the precursors for avilamycin biosynthesis and affected antibiotic synthesis.