A novel approach for myocardial regeneration with educated cord blood cells cocultured with cells from brown adipose tissue

Umbilical cord blood (CB) is a promising source for regeneration therapy in humans. Recently, it was shown that CB was a source of mesenchymal stem cells as well as hematopoietic stem cells, and further that the mesenchymal stem cells could differentiate into a number of cells types of mesenchymal l...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-02, Vol.353 (1), p.182-188
Main Authors: Yamada, Yoshihiro, Yokoyama, Shin-ichiro, Fukuda, Noboru, Kidoya, Hiroyasu, Huang, Xiao-Yong, Naitoh, Hisamichi, Satoh, Naoyuki, Takakura, Nobuyuki
Format: Article
Language:English
Subjects:
Adipocytes - transplantation
Adipose tissue
Adipose Tissue, Brown - cytology
Adipose Tissue, Brown - transplantation
Animals
Animals, Newborn
Cell fusion
Cells, Cultured
Coculture Techniques - methods
Cord blood
Cord Blood Stem Cell Transplantation - methods
Guided Tissue Regeneration - methods
Mice
Mice, Inbred C57BL
Myocardial Infarction - pathology
Myocardial Infarction - surgery
Myocardial regeneration
Rats
Rats, Nude
Stem cell
Treatment Outcome
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Summary:Umbilical cord blood (CB) is a promising source for regeneration therapy in humans. Recently, it was shown that CB was a source of mesenchymal stem cells as well as hematopoietic stem cells, and further that the mesenchymal stem cells could differentiate into a number of cells types of mesenchymal lineage, such as cardiomyocytes (CMs), osteocytes, chondrocytes, and fat cells. Previously, we reported that brown adipose tissue derived cells (BATDCs) differentiated into CMs and these CMs could adapt functionally to repair regions of myocardial infarction. In this study, we examined whether CB mononuclear cells (CBMNCs) could effectively differentiate into CMs by coculturing them with BATDCs and determined which population among CBMNCs differentiated into CMs. The results show that BATDCs effectively induced CBMNCs that were non-hematopoietic stem cells (HSCs) (educated CB cells: e-CBCs) into CMs in vitro. E-CBCs reconstituted infarcted myocardium more effectively than non-educated CBMNCs or CD34-positive HSCs. Moreover, we found that e-CBCs after 3 days coculturing with BATDCs induced the most effective regeneration for impaired CMs. This suggests that e-CBCs have a high potential to differentiate into CMs and that adequate timing of transplantation supports a high efficiency for CM regeneration. This strategy might be a promising therapy for human cardiac disease.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.12.017