Screening for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala koidz

• Published in: Journal of ethnopharmacology 2007-11, Vol.114 (2), p.212-217
• Main Authors: Li, Cui-Qin, He, Lang-Chong, Dong, Hai-Yan, Jin, Ju-Qing
• Format: Article
• Language: English
• Subjects: 14-Acetoxy-12-senecioyloxytetradeca-2 E,8 E,10 E-trien-4,6-diyn-1-ol; Acetic Acid - pharmacology; animal disease models; Animals; Anti-inflammation; anti-inflammatory activity; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Atractylenolide I; Atractylodes; Atractylodes - chemistry; Atractylodes macrocephala; Atractylodes macrocephala Koidz (Compositae); Biological and medical sciences; Capillary Permeability - drug effects; Carrageenan; Cell Membrane - drug effects; Cell membrane chromatography; chemical constituents of plants; Chromatography; Cotton Fiber; Dexamethasone - pharmacology; Drug Evaluation, Preclinical; Edema - chemically induced; Edema - prevention & control; Female; General pharmacology; Granuloma - pathology; Granuloma - prevention & control; in vivo studies; inflammation; leukocytes; Leukocytes - chemistry; Leukocytes - drug effects; Magnetic Resonance Spectroscopy; Male; Medical sciences; medicinal plants; mice; Oriental traditional medicine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; plant extracts; Plant Extracts - pharmacology; Plant Roots - chemistry; Rabbits; rhizomes; screening
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.08.002

The aim of this study was to screen for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala Koidz. The rhizomes of Atractylodes macrocephala were treated with supercritical CO 2 fluid and the extract was separated by normal-phase and reverse-phase column chromatography. The separated samples were screened with white blood cell membrane (WBCM) chromatography (WBCM-C). The anti-inflammatory effects of these fractions and components were tested pharmacologically in vivo. The results indicated that the retention characteristics of the petrol-ether (1:1, v/v) fraction (BZC-2) of the supercritical CO 2 extract, the atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2 E,8 E,10 E-trien-4, 6-diyn-1-ol isolated from BZC-2 as active fractions and components were similar to that of dexamethasone in WBCM-C. Therefore, they may act on WBCM and its receptors. BZC-2 has shown anti-inflammatory effects in acute and chronic inflammation models in rats and mice. Oral administration of atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2 E,8 E,10 E-trien-4,6-diyn-1-ol produced significant anti-inflammatory effects in acute and chronic inflammation models in mice. The screening results with WBCM-C were correlated significantly with pharmacological effects in vivo. Atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2 E,8 E,10 E-trien-4,6-diyn-1-ol were the main components of Atractylodes macrocephala that were effective as anti-inflammatory agents.