A novel transforming growth factor-β superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout ( Oncorhynchus mykiss)

Our understanding of the molecular mechanisms of primordial germ cell (PGC) proliferation in fish is rudimentary, but it is thought to be controlled by the surrounding somatic cells. We assumed that growth factors that are specifically involved in PGC proliferation are expressed predominantly in the...

Full description

Saved in:
Bibliographic Details
Published in:Developmental biology 2007, Vol.301 (1), p.266-275
Main Authors: Sawatari, Etsuko, Shikina, Shinya, Takeuchi, Toshio, Yoshizaki, Goro
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Brackish
Cytokine
DNA, Complementary
Freshwater
Germ Cells - cytology
Germ Cells - metabolism
Immunohistochemistry
In Situ Hybridization
Male
Marine
Molecular Sequence Data
Oligonucleotides, Antisense
Oncorhynchus mykiss
Oncorhynchus mykiss - embryology
PGC
Phylogeny
Sertoli cell
Spermatogonia
Spermatogonia - metabolism
Transforming Growth Factor beta - genetics
Trout
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Our understanding of the molecular mechanisms of primordial germ cell (PGC) proliferation in fish is rudimentary, but it is thought to be controlled by the surrounding somatic cells. We assumed that growth factors that are specifically involved in PGC proliferation are expressed predominantly in the surrounding genital ridge somatic cells. In order to isolate these growth factors, we compiled a complementary DNA (cDNA) subtractive library using cDNA from the genital ridges of 40-dpf rainbow trout embryos as the tester and cDNA from embryos without genital ridges as the driver. This approach identified a novel cytokine, designated gonadal soma-derived growth factor (GSDF), which is a member of the transforming growth factor (TGF)-β superfamily. GSDF was expressed in the genital ridge somatic cells surrounding the PGCs during embryogenesis, and in both the granulosa and Sertoli cells at later stages. Inhibition of GSDF translation by antisense oligonucleotides suppressed PGC proliferation. Moreover, isolated testicular cells that were cultured with recombinant GSDF demonstrated dose-dependent proliferation of type-A spermatogonia; this effect was completely blocked by antiserum against GSDF. These results denote that GSDF, a novel member of the TGF-β superfamily, plays an important role for proliferation of PGC and spermatogonia.
ISSN:0012-1606
1095-564X
DOI:10.1016/j.ydbio.2006.10.001