Comparison of universal detectors for high-temperature micro liquid chromatography

This study compares, through micro high-temperature liquid chromatography (μHTLC), three commercial universal detectors that allow a direct detection of lipids. The detectors are: the charged aerosol detector (CAD), the evaporative light-scattering detector (ELSD) and the ion trap mass spectrometer...

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Bibliographic Details
Published in:Journal of Chromatography A 2007-11, Vol.1170 (1), p.52-61
Main Authors: Hazotte, A., Libong, D., Matoga, M., Chaminade, P.
Format: Article
Language:English
Subjects:
Aerosols - chemistry
Analytical chemistry
Ceramides - analysis
Charged aerosol detection
Chemistry
Cholesterol - analysis
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Evaporative light-scattering detection
Exact sciences and technology
High-temperature liquid chromatography
Hot Temperature
Light
Lipids
Lipids - analysis
Mass spectrometry, APCI, ESI
Micro column liquid chromatography
Microfluidics - methods
Other chromatographic methods
Scattering, Radiation
Sensitivity and Specificity
Solvents - chemistry
Spectrophotometry - instrumentation
Spectrophotometry - methods
Squalene - analysis
Temperature
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Summary:This study compares, through micro high-temperature liquid chromatography (μHTLC), three commercial universal detectors that allow a direct detection of lipids. The detectors are: the charged aerosol detector (CAD), the evaporative light-scattering detector (ELSD) and the ion trap mass spectrometer with atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI) sources (APCI-MS and ESI-MS). This study shows the feasibility to use the high temperature with these detectors and hybrid behavior between concentration and mass flow rate detector in μHTLC. The detectors were compared in terms of response intensity, linearity and limit of detection for different high temperatures. The charged aerosol detector shows a linear response from 5 to 500 μg/mL and the correlation coefficients ( r 2) obtained for squalene, cholesterol and ceramide IIIB exceed 0.99.
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.09.008