A modified model of hindlimb osteomyocutaneous flap for the study of tolerance to composite tissue allografts

Composite tissue allotransplantation (CTA) is the new frontier in transplantation. More than 25 hand allograft transplants have been performed worldwide, and the feasibility has been well established. The classical experimental model of CTA involves rat orthotopic hindlimb transplantation, a time‐co...

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Bibliographic Details
Published in:Microsurgery 2007, Vol.27 (7), p.630-636
Main Authors: Adamson, Larry A., Huang, Wei-Chao, Breidenbach, Warren C., Rahhal, Dina, Xu, Hong, Huang, Yiming, Pidwell, Diane J., Wei, Fu-Chan, Tobin, Gordon, Ildstad, Suzanne T.
Format: Article
Language:English
Subjects:
Animals
Disease Models, Animal
Graft Survival
Hindlimb - transplantation
Male
Rats
Rats, Inbred ACI
Rats, Wistar
Surgical Flaps - immunology
Tissue Transplantation - methods
Transplantation Chimera
Transplantation Tolerance - physiology
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Summary:Composite tissue allotransplantation (CTA) is the new frontier in transplantation. More than 25 hand allograft transplants have been performed worldwide, and the feasibility has been well established. The classical experimental model of CTA involves rat orthotopic hindlimb transplantation, a time‐consuming procedure associated with high mortality and morbidity. We describe a rat heterotopic osteomyocutaneous flap that serves as a nonfunctional CTA, allowing the study of tolerance induction to a highly antigenic vascularized allograft of bone, muscle, and skin while minimizing the morbidity and mortality of full hind limb transplantation. In the present studies, we explored whether establishing chimerism by nonmyeloablative conditioning would induce tolerance to CTA. When compared with the classic hind limb transplantation model, these results demonstrate that our heterotopic hind limb flap is less morbid and as an effective experimental model for the study of CTA tolerance. © 2007 Wiley‐Liss, Inc. Microsurgery, 2007.
ISSN:0738-1085
1098-2752
DOI:10.1002/micr.20414