Increased toll-like receptor 9 expression in cervical neoplasia

Toll‐like receptors (TLRs) recognize pathogen‐associated molecular patterns (PAMPs) and enable innate immune responses. Although TLR9 has been previously considered to be expressed only in immune cells, there is now increasing evidence that TLR9 expression is present in nonimmune cells as well. In t...

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Published in:Molecular carcinogenesis 2007-11, Vol.46 (11), p.941-947
Main Authors: Lee, Jeong-Won, Choi, Jung-Joo, Seo, Eun Sung, Kim, Mi Jin, Kim, Woo Young, Choi, Chel Hun, Kim, Tae-Joong, Kim, Byoung-Gie, Song, Sang Yong, Bae, Duk-Soo
Format: Article
Language:English
Subjects:
carcinoma
Carcinoma, Squamous Cell
Cervical Intraepithelial Neoplasia
cervix neoplasms
Cervix Uteri - metabolism
Disease Progression
Female
Humans
Immunohistochemistry
Reverse Transcriptase Polymerase Chain Reaction
squamous cell
toll-like receptor
Toll-Like Receptor 9 - metabolism
Uterine Cervical Neoplasms - metabolism
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Summary:Toll‐like receptors (TLRs) recognize pathogen‐associated molecular patterns (PAMPs) and enable innate immune responses. Although TLR9 has been previously considered to be expressed only in immune cells, there is now increasing evidence that TLR9 expression is present in nonimmune cells as well. In this study, we undertook to determine whether TLR9 expression was associated with disease progression in cervical neoplasia. TLR9 expression was evaluated by immunohistochemistry in 55 formalin‐fixed paraffin‐embedded cervical tissues; nine normal cervical specimens, 10 low‐grade cervical intraepithelial neoplasias (CINs), 12 high‐grade CINs, and 24 invasive squamous cell carcinomas (ISCCs). In addition, TLR9 expression was evaluated, at the RNA level, in fresh frozen cervical carcinoma tissues by real‐time quantitative RT‐PCR. Immunohistochemical staining showed that TLR9 expression was undetectable (55.6%) or weak (44.4%) in normal cervical squamous epithelial tissues, however, variable staining was observed in the basal layer of all normal endocervical glands. TLR9 expression, which was mainly observed as cytoplasmic staining, gradually increased in accordance with the histopathological grade in the following order: low‐grade CIN 
ISSN:0899-1987
1098-2744
DOI:10.1002/mc.20325