Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity

Background:  The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody‐induced depletion of specific subtypes of im...

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Bibliographic Details
Published in:Contact dermatitis 2007-11, Vol.57 (5), p.300-308
Main Authors: Larsen, Jeppe Madura, Geisler, Carsten, Nielsen, Martin Weiss, Boding, Lasse, Von Essen, Marina, Hansen, Ann Kathrine, Skov, Lone, Bonefeld, Charlotte Menné
Format: Article
Language:English
Subjects:
activation
Allergens - immunology
Allergic diseases
Animals
Antibodies - immunology
Biological and medical sciences
contact hypersensitivity
Dermatitis, Allergic Contact - immunology
Dinitrofluorobenzene - immunology
Female
Flow Cytometry
Immunopathology
local lymph node assay
Lymph Nodes - cytology
Lymph Nodes - immunology
Lymph Nodes - physiology
Lymphocyte Activation
lymphocytes
Medical sciences
Mice
Mice, Inbred C57BL
Oxazolone - immunology
Skin allergic diseases. Stinging insect allergies
T-Lymphocytes - immunology
T-Lymphocytes - physiology
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Summary:Background:  The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody‐induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations. Objectives:  To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals. Patients/Methods:  Mice were sensitized and/or challenged with 2,4‐dinitrofluorobenzene or oxazolone. Using multi‐parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes. Results:  The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system. Conclusions:  Our study support recent findings that several cell types seem to be involved in CHS.
ISSN:0105-1873
1600-0536
DOI:10.1111/j.1600-0536.2007.01230.x