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Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity
Background: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody‐induced depletion of specific subtypes of im...
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| Published in: | Contact dermatitis 2007-11, Vol.57 (5), p.300-308 |
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| Main Authors: | , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c5320-4b29a616043c624a0011e79d5d092d1c2e1d6e9b2edc883e00974902591fbecb3 |
| container_end_page | 308 |
| container_issue | 5 |
| container_start_page | 300 |
| container_title | Contact dermatitis |
| container_volume | 57 |
| creator | Larsen, Jeppe Madura Geisler, Carsten Nielsen, Martin Weiss Boding, Lasse Von Essen, Marina Hansen, Ann Kathrine Skov, Lone Bonefeld, Charlotte Menné |
| description | Background: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody‐induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.
Objectives: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.
Patients/Methods: Mice were sensitized and/or challenged with 2,4‐dinitrofluorobenzene or oxazolone. Using multi‐parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.
Results: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.
Conclusions: Our study support recent findings that several cell types seem to be involved in CHS. |
| doi_str_mv | 10.1111/j.1600-0536.2007.01230.x |
| format | article |
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Objectives: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.
Patients/Methods: Mice were sensitized and/or challenged with 2,4‐dinitrofluorobenzene or oxazolone. Using multi‐parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.
Results: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.
Conclusions: Our study support recent findings that several cell types seem to be involved in CHS.</description><identifier>ISSN: 0105-1873</identifier><identifier>EISSN: 1600-0536</identifier><identifier>DOI: 10.1111/j.1600-0536.2007.01230.x</identifier><identifier>PMID: 17937744</identifier><identifier>CODEN: CODEDG</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>activation ; Allergens - immunology ; Allergic diseases ; Animals ; Antibodies - immunology ; Biological and medical sciences ; contact hypersensitivity ; Dermatitis, Allergic Contact - immunology ; Dinitrofluorobenzene - immunology ; Female ; Flow Cytometry ; Immunopathology ; local lymph node assay ; Lymph Nodes - cytology ; Lymph Nodes - immunology ; Lymph Nodes - physiology ; Lymphocyte Activation ; lymphocytes ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Oxazolone - immunology ; Skin allergic diseases. Stinging insect allergies ; T-Lymphocytes - immunology ; T-Lymphocytes - physiology</subject><ispartof>Contact dermatitis, 2007-11, Vol.57 (5), p.300-308</ispartof><rights>2007 Blackwell Munksgaard</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5320-4b29a616043c624a0011e79d5d092d1c2e1d6e9b2edc883e00974902591fbecb3</citedby><cites>FETCH-LOGICAL-c5320-4b29a616043c624a0011e79d5d092d1c2e1d6e9b2edc883e00974902591fbecb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19149007$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17937744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Larsen, Jeppe Madura</creatorcontrib><creatorcontrib>Geisler, Carsten</creatorcontrib><creatorcontrib>Nielsen, Martin Weiss</creatorcontrib><creatorcontrib>Boding, Lasse</creatorcontrib><creatorcontrib>Von Essen, Marina</creatorcontrib><creatorcontrib>Hansen, Ann Kathrine</creatorcontrib><creatorcontrib>Skov, Lone</creatorcontrib><creatorcontrib>Bonefeld, Charlotte Menné</creatorcontrib><title>Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity</title><title>Contact dermatitis</title><addtitle>Contact Dermatitis</addtitle><description>Background: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody‐induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.
Objectives: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.
Patients/Methods: Mice were sensitized and/or challenged with 2,4‐dinitrofluorobenzene or oxazolone. Using multi‐parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.
Results: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.
Conclusions: Our study support recent findings that several cell types seem to be involved in CHS.</description><subject>activation</subject><subject>Allergens - immunology</subject><subject>Allergic diseases</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Biological and medical sciences</subject><subject>contact hypersensitivity</subject><subject>Dermatitis, Allergic Contact - immunology</subject><subject>Dinitrofluorobenzene - immunology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Immunopathology</subject><subject>local lymph node assay</subject><subject>Lymph Nodes - cytology</subject><subject>Lymph Nodes - immunology</subject><subject>Lymph Nodes - physiology</subject><subject>Lymphocyte Activation</subject><subject>lymphocytes</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Oxazolone - immunology</subject><subject>Skin allergic diseases. Stinging insect allergies</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - physiology</subject><issn>0105-1873</issn><issn>1600-0536</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkU2P1CAAhonRuOPqXzBc9NbKRwvl4MGMuppMdqPR6I1QoA5jSyu0OvXXS22ze9ReKPA8wJsXAIhRjtP34pRjhlCGSspyghDPESYU5ed7YHe7cR_sEEZlhitOL8CjGE8IYVaQ6iG4wFxQzotiB6a9bdupVQGa2avO6Qidh-PRQhOU885_g-3cDUfoe2MjNFNYlqL10Y3utxpd76HyBtrWaTeu8-GoYmL7Burej0qP8DgPNmzSTzfOj8GDRrXRPtnGS_D57ZtP-3fZ4ebq_f7VIdMlJSgraiIUS4kKqhkpVAqALRemNEgQgzWx2DAramKNripqERK8EIiUAje11TW9BM_Xc4fQ_5hsHGXnok6Jlbf9FCWrqCgoZ_8ECSorVpYigdUK6tDHGGwjh-A6FWaJkVy6kSe5VCCXCuTSjfzbjTwn9el2x1R31tyJWxkJeLYBKmrVNkF57eIdJ3AKh3jiXq7cL9fa-b8fIPc3r5e_5Ger7-Joz7e-Ct8l45SX8sv1lWT0-uOHr-QgCf0Dowa6yQ</recordid><startdate>200711</startdate><enddate>200711</enddate><creator>Larsen, Jeppe Madura</creator><creator>Geisler, Carsten</creator><creator>Nielsen, Martin Weiss</creator><creator>Boding, Lasse</creator><creator>Von Essen, Marina</creator><creator>Hansen, Ann Kathrine</creator><creator>Skov, Lone</creator><creator>Bonefeld, Charlotte Menné</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200711</creationdate><title>Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity</title><author>Larsen, Jeppe Madura ; Geisler, Carsten ; Nielsen, Martin Weiss ; Boding, Lasse ; Von Essen, Marina ; Hansen, Ann Kathrine ; Skov, Lone ; Bonefeld, Charlotte Menné</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5320-4b29a616043c624a0011e79d5d092d1c2e1d6e9b2edc883e00974902591fbecb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>activation</topic><topic>Allergens - immunology</topic><topic>Allergic diseases</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Biological and medical sciences</topic><topic>contact hypersensitivity</topic><topic>Dermatitis, Allergic Contact - immunology</topic><topic>Dinitrofluorobenzene - immunology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Immunopathology</topic><topic>local lymph node assay</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - immunology</topic><topic>Lymph Nodes - physiology</topic><topic>Lymphocyte Activation</topic><topic>lymphocytes</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Oxazolone - immunology</topic><topic>Skin allergic diseases. Stinging insect allergies</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Larsen, Jeppe Madura</creatorcontrib><creatorcontrib>Geisler, Carsten</creatorcontrib><creatorcontrib>Nielsen, Martin Weiss</creatorcontrib><creatorcontrib>Boding, Lasse</creatorcontrib><creatorcontrib>Von Essen, Marina</creatorcontrib><creatorcontrib>Hansen, Ann Kathrine</creatorcontrib><creatorcontrib>Skov, Lone</creatorcontrib><creatorcontrib>Bonefeld, Charlotte Menné</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Contact dermatitis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Larsen, Jeppe Madura</au><au>Geisler, Carsten</au><au>Nielsen, Martin Weiss</au><au>Boding, Lasse</au><au>Von Essen, Marina</au><au>Hansen, Ann Kathrine</au><au>Skov, Lone</au><au>Bonefeld, Charlotte Menné</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity</atitle><jtitle>Contact dermatitis</jtitle><addtitle>Contact Dermatitis</addtitle><date>2007-11</date><risdate>2007</risdate><volume>57</volume><issue>5</issue><spage>300</spage><epage>308</epage><pages>300-308</pages><issn>0105-1873</issn><eissn>1600-0536</eissn><coden>CODEDG</coden><abstract>Background: The different role of various immunological effector cells in contact hypersensitivity (CHS) is receiving increased attention. During the past decade, the involvement of different cell types in CHS has been investigated by the use of antibody‐induced depletion of specific subtypes of immunological cells and by studying knockout mice lacking one or more of these immunological cell populations.
Objectives: To develop a method for studying the collective cellular dynamics of immune cells in the draining lymph nodes during CHS in intact animals.
Patients/Methods: Mice were sensitized and/or challenged with 2,4‐dinitrofluorobenzene or oxazolone. Using multi‐parameter flow cytometry we determined the proliferation, activation state, and absolute number of helper T cells, cytotoxic T cells, B cells, and natural killer cells in the draining lymph nodes.
Results: The presented method can be applied to evaluate the effect of different contact allergens on various cell populations of the immune system.
Conclusions: Our study support recent findings that several cell types seem to be involved in CHS.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17937744</pmid><doi>10.1111/j.1600-0536.2007.01230.x</doi><tpages>9</tpages></addata></record> |
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| ispartof | Contact dermatitis, 2007-11, Vol.57 (5), p.300-308 |
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| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | activation Allergens - immunology Allergic diseases Animals Antibodies - immunology Biological and medical sciences contact hypersensitivity Dermatitis, Allergic Contact - immunology Dinitrofluorobenzene - immunology Female Flow Cytometry Immunopathology local lymph node assay Lymph Nodes - cytology Lymph Nodes - immunology Lymph Nodes - physiology Lymphocyte Activation lymphocytes Medical sciences Mice Mice, Inbred C57BL Oxazolone - immunology Skin allergic diseases. Stinging insect allergies T-Lymphocytes - immunology T-Lymphocytes - physiology |
| title | Cellular dynamics in the draining lymph nodes during sensitization and elicitation phases of contact hypersensitivity |
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