Single-molecule detection for femtomolar quantification of proteins in heterogeneous immunoassays

Flow velocity and data acquisition rates can be matched to provide highly accurate quantification, even for rapid analyses on the order of seconds per sample, and analysis time can easily be extended to provide increased detection efficiency of rare targets. Because the interrogation zone is on the...

Full description

Saved in:
Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2006-11, Vol.52 (11), p.2172-2175
Main Authors: Nalefski, Eric A, D'Antoni, Christina M, Ferrell, Evan P, Lloyd, Janice A, Qiu, Haoqun, Harris, John L, Whitney, Duncan H
Format: Article
Language:English
Subjects:
Analytical chemistry
Antibodies, Monoclonal
Data acquisition
Flow velocity
Fluorescence
Follicle Stimulating Hormone - analysis
Follicle Stimulating Hormone - immunology
Humans
Immunoassay
Immunoassay - methods
Immunoassays
Molecules
Monoclonal antibodies
Sample size
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Flow velocity and data acquisition rates can be matched to provide highly accurate quantification, even for rapid analyses on the order of seconds per sample, and analysis time can easily be extended to provide increased detection efficiency of rare targets. Because the interrogation zone is on the order of femtoliters, the inner diameter tit' the sample delivery microcapillary is on the order of tens of micrometers, suitable counting statistics are achieved in seconds, and only small volumes of captured and released reporter antibodies are required for analysis.
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2006.072850