Hydrogen Bonding versus Ion Pairing in Polyelectrolyte Multilayers with Homopolynucleotides

Homopolynucleotidespoly(adenylic acid), poly(A), and poly(uridylic acid), poly(U)were assembled, layer-by-layer, into thin films with poly(ethylenimine), PEI. Various combinations and sequences of polynucleotide and PEI were used to highlight contributions of electrostatic versus hydrogen bonding...

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Bibliographic Details
Published in:Biomacromolecules 2007-01, Vol.8 (1), p.59-64
Main Authors: Markarian, Marie Z, Moussallem, Maroun D, Jomaa, Houssam W, Schlenoff, Joseph B
Format: Article
Language:English
Subjects:
Adsorption
Applied sciences
Chemical Phenomena
Chemistry, Physical
Circular Dichroism
Dose-Response Relationship, Drug
Electrolytes
Exact sciences and technology
Hydrogen Bonding
Ions
Microscopy, Atomic Force
Molecular Conformation
Nucleotides - chemistry
Organic polymers
Physicochemistry of polymers
Polyethyleneimine - chemistry
Polynucleotides - chemistry
Properties and characterization
Salts - chemistry
Structure, morphology and analysis
Surface Properties
Time Factors
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Summary:Homopolynucleotidespoly(adenylic acid), poly(A), and poly(uridylic acid), poly(U)were assembled, layer-by-layer, into thin films with poly(ethylenimine), PEI. Various combinations and sequences of polynucleotide and PEI were used to highlight contributions of electrostatic versus hydrogen bonding as driving forces for multilayer build-up. Assembly of alternating poly(A) and poly(U) failed to yield growing films, due to excessively strong interactions between these complimentary strands. The surface morphology of multilayers depended on the deposition order and whether films had been annealed by salt. Films assembled from preformed A/U duplexes (having high persistence lengths) were very smooth. Individual adsorption steps, followed by optical waveguide light-mode spectroscopy, showed that only complementary polynucleotides adsorb by H-bonding to the surface of a growing multilayer. In contrast to behavior usually observed for polyelectrolyte multilayer build-up, the films decreased in thickness with increasing salt concentration.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm0604909