Determination of doxycycline in pharmaceuticals and human urine by micellar electrokinetic capillary chromatography

Micellar electrokinetic capillary chromatography (MEKC) was performed at 25 degrees C and 30 kV (under a pressure of 15 mbar), using 30 mM borate buffer containing 60 mM sodium dodecysulfate (SDS) and 5% (v/v) methanol as background electrolyte (pH 9.0) to determine doxycycline. UV detection was at...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2007-01, Vol.387 (2), p.695-701
Main Authors: Injac, Rade, Kac, Javor, Kreft, Samo, Strukelj, Borut
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents
Chromatography, Micellar Electrokinetic Capillary - methods
Chromatography, Micellar Electrokinetic Capillary - standards
Doxycycline - analysis
Doxycycline - urine
Humans
Pharmaceutical Preparations - analysis
Reproducibility of Results
Sensitivity and Specificity
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Summary:Micellar electrokinetic capillary chromatography (MEKC) was performed at 25 degrees C and 30 kV (under a pressure of 15 mbar), using 30 mM borate buffer containing 60 mM sodium dodecysulfate (SDS) and 5% (v/v) methanol as background electrolyte (pH 9.0) to determine doxycycline. UV detection was at 350 nm. The method was shown to be specific, accurate (recovery was 100.3 +/- 1.0%), linear over the tested range (correlation coefficient 0.9995) and precise (RSD
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-006-0900-9