Early detection of viral excretion from experimentally infected goats with peste-des-petits ruminants virus

• Published in: Preventive veterinary medicine 2007-01, Vol.78 (1), p.85-88
• Main Authors: Couacy-Hymann, E., Bodjo, S.C., Danho, T., Koffi, M.Y., Libeau, G., Diallo, A.
• Format: Article
• Language: English
• Subjects: Africa South of the Sahara; Animals; Body Temperature; carrier state; Carrier State - veterinary; Carrier State - virology; disease detection; disease diagnosis; disease vectors; Epidemiology; eyes; Goat; goat diseases; Goat Diseases - transmission; Goat Diseases - virology; Goats; incubation period; infection; microbial detection; Morbillivirus; nasal cavity; pathogenesis; peste des petits ruminants; Peste-des-petits-ruminants (PPR); Peste-des-Petits-Ruminants - transmission; Peste-des-Petits-Ruminants - veterinary; Peste-des-Petits-Ruminants - virology; Peste-des-petits-ruminants virus; Peste-des-petits-ruminants virus - genetics; Peste-des-petits-ruminants virus - growth & development; PPRV; Random Allocation; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Viral - chemistry; RNA, Viral - genetics; saliva; signs and symptoms (animals and humans); Virus Shedding; virus transmission
• ISSN: 0167-5877; 1873-1716
• DOI: 10.1016/j.prevetmed.2006.09.003

We observed 15 goats for 9 days after subcutaneous infection with 10 3 TCID 50 with isolates of peste-des-petits ruminants virus from Africa and India and five concurrent, uninfected control goats. Typical clinical signs of the infection were present in all 15 infected goats by day 8 and in most by day 6 and some signs were present by day 4. However, 6 out of 15 goats already have detectable virus shedding by day 3 and four more were shedding by day 4 and every goat had virus shedding for at least 1 day before the recognition of clinical signs. This experiment indicates that incubatory carriers therefore might play a role in the transmission of PPRV among small ruminants.