Co-Utilization of Canola Oil and Glucose on the Production of a Surfactant by Candida lipolytica

Candida lipolytica synthesized a surfactant in a cultivation medium supplemented with canola oil and glucose as carbon sources. Measurements of biosurfactant production and surface tension indicated that the biosurfactant was produced at 48 h of fermentation. The surface-active species is constitute...

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Bibliographic Details
Published in:Current microbiology 2007, Vol.54 (1), p.68-73
Main Authors: Sarubbo, Leonie A, Farias, Charles B. B, Campos-Takaki, Galba Maria
Format: Article
Language:English
Subjects:
Bacteria
Candida - metabolism
Candida lipolytica
Canola Oil
Carbon sources
Chloroform
Fatty Acids, Monounsaturated - metabolism
Fermentation
Glucose - metabolism
Hydrogen-Ion Concentration
Industrial Microbiology
Sodium Chloride - metabolism
Surface tension
Surface-Active Agents - chemistry
Surface-Active Agents - isolation & purification
Surface-Active Agents - metabolism
Surfactants
Temperature
Vegetable oils
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Summary:Candida lipolytica synthesized a surfactant in a cultivation medium supplemented with canola oil and glucose as carbon sources. Measurements of biosurfactant production and surface tension indicated that the biosurfactant was produced at 48 h of fermentation. The surface-active species is constituted by the protein-lipid-polysaccharide complex in nature. The cell-free broth was particularly influenced by the addition of salt, the pH and temperature depending on the emulsified substrate (hexadecane or a vegetable oil). After comparison between ethyl acetate and mixtures of chloroform and methanol as solvent systems for surfactant recovery, it was found that ethyl acetate was able to extract crude surfactant material with high product recovery (8.0 g/L). The isolated biosurfactant decreased the surface tension to values of 30 mN/m at the critical micelle concentration. Emulsification properties of the biosurfactant produced were compared to those of commercial emulsifiers and other microbial surfactants.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-006-0412-z