Identification of proteins undergoing expression level modifications in WI-38 SV40 fibroblasts overexpressing methionine sulfoxide reductase A

Methionine sulfoxide reductase A overexpressing WI-38 SV40 human fibroblasts have been previously shown to exhibit higher resistance to oxidative stress by decreasing intracellular reactive oxygen species content and oxidative damage to proteins [C.R. Picot, I. Petropoulos, M. Perichon, M. Moreau, C...

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Bibliographic Details
Published in:Biochimie 2007-11, Vol.89 (11), p.1388-1395
Main Authors: Cabreiro, F., Picot, C.R., Perichon, M., Mary, J., Friguet, B., Petropoulos, I.
Format: Article
Language:English
Subjects:
Cell Line, Transformed
Clone Cells - drug effects
Down-Regulation - drug effects
Electrophoresis, Gel, Two-Dimensional
Fibroblasts - enzymology
Fibroblasts - metabolism
Gas Chromatography-Mass Spectrometry
Gene Expression
Gene Expression Profiling
Humans
Hydrogen Peroxide - pharmacology
Mass spectrometry
Methionine sulfoxide reductase A
Methionine Sulfoxide Reductases
Oxidative stress
Oxidative Stress - drug effects
Oxidoreductases - genetics
Oxidoreductases - metabolism
Proteome - metabolism
Proteomics
Simian virus 40
Up-Regulation - drug effects
WI-38 SV40 fibroblasts
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Summary:Methionine sulfoxide reductase A overexpressing WI-38 SV40 human fibroblasts have been previously shown to exhibit higher resistance to oxidative stress by decreasing intracellular reactive oxygen species content and oxidative damage to proteins [C.R. Picot, I. Petropoulos, M. Perichon, M. Moreau, C. Nizard, B. Friguet, Overexpression of MsrA protects WI-38 SV40 human fibroblasts against H 2O 2-mediated oxidative stress, Free Radic Biol Med 39 (2005) 1332-1341]. In order to get further insight into the molecular mechanisms underlying this resistance to oxidative stress, proteins that are differentially expressed in methionine sulfoxide reductase A overexpressing cells were identified by 2D gel and Western blot quantitative analyses. Five proteins were shown to be differentially expressed and were identified by mass spectrometry, some of them were related to either cellular protection against oxidative stress, apoptosis or premature ageing.
ISSN:0300-9084
1638-6183
DOI:10.1016/j.biochi.2007.05.005