Analysis of Protein Expression Profiles of Halobacillus dabanensis D-8T Under Optimal and High Salinity Conditions

Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8T under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemaster[trade mark sign] 2D Platinum software. The molecul...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 2007, Vol.54 (1), p.20-26
Main Authors: Feng, De Qin, Yang, Li Fu, Lu, Wei Dong, Yang, Su Sheng
Format: Article
Language:English
Subjects:
Bacillaceae - genetics
Bacillaceae - metabolism
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Desorption
Down-Regulation
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation, Bacterial
Ionization
Isoelectric Point
Mass spectroscopy
Microbiology
Molecular Weight
Proteins
Proteomics - methods
Salinity
Salts
Sodium Chloride - analysis
Software
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8T under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemaster[trade mark sign] 2D Platinum software. The molecular masses of the majority of intracellular proteins were distributed in the range of 17.5 kDa-66 kDa and isoelectric points of 4.0-5.9. In total 133 protein spots were observed with a changed expression level under different salinity conditions. Sixty-two protein spots showed upregulation and 26 new protein spots were found under high salinity conditions, while 25 protein spots were downregulated and 20 spots disappeared. Twenty-seven proteins with a markedly changed expression in hypersaline environments were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF/MS) and MASCOT. A changed expression pattern was observed for proteins related to energy-producing pathways, stress regulators, and proteins involved in the survival of strain D-8T under high salt challenges. Many proteins play necessary roles in the adaptation to high salt or as a general stress protein, and some proteins are salt-stressed specific proteins that improve the capability of salt-tolerance of strain D-8T growth under extremely hypersaline condition.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-006-0221-4