Deduced amino acid sequences surrounding the fusion glycoprotein cleavage site and the carboxyl-terminus of haemagglutinin-neuraminidase protein of the avirulent thermostable vaccine strain I-2 of Newcastle disease virus

A single-tube RT-PCR technique generated a 387 bp or 300 bp cDNA amplicon covering the F(0) cleavage site or the carboxyl (C)-terminus of the HN gene, respectively, of Newcastle disease virus (NDV) strain I-2. Sequence analysis was used to deduce the amino acid sequences of the cleavage site of F pr...

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Bibliographic Details
Published in:Veterinary research communications 2007, Vol.31 (1), p.105-112
Main Authors: Wambura, P.N, Meers, J, Kattenbelt, J.A, Gould, A.R, Spradbrow, P.B
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
amino acid sequences
Animals
Chickens
complementary DNA
disease outbreaks
HN Protein - chemistry
HN Protein - genetics
HN Protein - metabolism
live vaccines
microbial genetics
Molecular Sequence Data
Newcastle disease virus
Newcastle disease virus - genetics
Newcastle disease virus - metabolism
nucleotide sequences
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - chemistry
RNA, Viral - genetics
sequence analysis
Sequence Analysis, DNA
strain differences
vaccine strains
vertebrate viruses
viral fusion proteins
Viral Fusion Proteins - chemistry
Viral Fusion Proteins - genetics
Viral Fusion Proteins - metabolism
virulence
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Summary:A single-tube RT-PCR technique generated a 387 bp or 300 bp cDNA amplicon covering the F(0) cleavage site or the carboxyl (C)-terminus of the HN gene, respectively, of Newcastle disease virus (NDV) strain I-2. Sequence analysis was used to deduce the amino acid sequences of the cleavage site of F protein and the C-terminus of HN protein, which were then compared with sequences for other NDV strains. The cleavage site of NDV strain I-2 had a sequence motif of (112) RKQGRLIG(119), consistent with an avirulent phenotype. Nucleotide sequencing and deduction of amino acids at the C-terminus of HN revealed that strain I-2 had a 7-amino-acid extension (VEILKDGVREARSSR. This differs from the virulent viruses that caused outbreaks of Newcastle disease in Australia in the 1930s and 1990s, which have HN extensions of 0 and 9 amino acids, respectively. Amino acid sequence analyses of the F and HN genes of strain I-2 confirmed its avirulent nature and its Australian origin.
ISSN:0165-7380
1573-7446
DOI:10.1007/s11259-006-3290-8