An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides

DNA was site-specifically conjugated to a substrate peptide of microbial transglutaminase fused to the N- or C-terminus of target proteins without the loss of the proteins' functions of interest.

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2007-01 (4), p.401-403
Main Authors: Tominaga, Jo, Kemori, Yoshinori, Tanaka, Yusuke, Maruyama, Tatsuo, Kamiya, Noriho, Goto, Masahiro
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Oligonucleotides - chemistry
Oligonucleotides - metabolism
Recombinant Proteins - analysis
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Transglutaminases - metabolism
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Description
Summary:DNA was site-specifically conjugated to a substrate peptide of microbial transglutaminase fused to the N- or C-terminus of target proteins without the loss of the proteins' functions of interest.
ISSN:1359-7345
1364-548X
DOI:10.1039/b613793h