Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage

Low molecular weight chitosan (LMWC) and chitooligosaccharides (COs), obtained by persulfate-induced free radical depolymerization of chitosan, showed scavenging of OH and O 2 - radicals, and offered protection against calf thymus DNA damage. Low molecular weight chitosan (LMWC) and chitooligosaccha...

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Bibliographic Details
Published in:Carbohydrate research 2007-02, Vol.342 (2), p.190-195
Main Authors: Harish Prashanth, Keelara V., Dharmesh, Shylaja M., Jagannatha Rao, Kosagi S., Tharanathan, Rudrapatnam N.
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Cattle
Chitosan
Chitosan - chemistry
Chitosan - metabolism
Circular Dichroism
Degradation products
DNA - metabolism
DNA condensation
DNA Damage - drug effects
Free Radical Scavengers - chemistry
Free Radical Scavengers - metabolism
Free radicals
Free Radicals - chemistry
Free Radicals - metabolism
Gene therapy
Hydroxides - chemistry
Hydroxides - metabolism
Molecular Weight
Transfection
Transition Temperature
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Summary:Low molecular weight chitosan (LMWC) and chitooligosaccharides (COs), obtained by persulfate-induced free radical depolymerization of chitosan, showed scavenging of OH and O 2 - radicals, and offered protection against calf thymus DNA damage. Low molecular weight chitosan (LMWC) and chitooligosaccharides (COs), obtained by persulfate-induced depolymerization of chitosan showed scavenging of OH and O 2 - radicals and offered protection against calf thymus DNA damage. Over 85% inhibition of free radicals and DNA protection were observed. LMWC (0.05 μmol) showed a strong inhibitory activity compared to COs (3.6 μmol). Further, LMWC showed calf thymus DNA condensation reversibly giving stability, as evident from CD, TEM and melting curves ( T m). A fluorescence study suggests the binding of LMWC in the minor groove, forming H-bonds to the backbone phosphates without distorting the double helix structure.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2006.11.010