Identification of four alcohol oxidases from methylotrophic yeasts

Three yeast strains capable of utilizing methanol as sole carbon and energy source were isolated. Two were classified as Candida boidinii, while the third belonged in the genus Pichia. From these three strains, four alcohol oxidases genes were identified and the sequences of the coding regions were...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2005-06, Vol.22 (8), p.669-676
Main Authors: Szamecz, Béla, Urbán, Gabriella, Rubiera, Roger, Kucsera, Judit, Dorgai, László
Format: Article
Language:English
Subjects:
alcohol oxidase
Alcohol Oxidoreductases - chemistry
Alcohol Oxidoreductases - genetics
Alcohol Oxidoreductases - metabolism
Amino Acid Sequence
Candida - classification
Candida - enzymology
Candida - genetics
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - metabolism
Methanol - metabolism
methylotrophic yeast
Molecular Sequence Data
Phylogeny
Pichia - classification
Pichia - enzymology
Pichia - genetics
Sequence Analysis, DNA
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Summary:Three yeast strains capable of utilizing methanol as sole carbon and energy source were isolated. Two were classified as Candida boidinii, while the third belonged in the genus Pichia. From these three strains, four alcohol oxidases genes were identified and the sequences of the coding regions were determined: one from each Candida boidinii (aox0673 and aox0680) and two from Pichia sp. 159 (aoxA and aoxB). Methanol induces both alcohol oxidases in Pichia sp. 159: the levels of aoxA and aoxB mRNA reach about 100% and 300%, respectively, of that of his4 mRNA. aoxA, but not aoxB, is expressed at a low level in the presence of glucose. The newly described alcohol oxidases have proper dinucleotide binding sites and PTS1‐like C‐terminal tripeptides, identified as important elements for peroxisomal localization. GenBank Accession Nos: Candida boidinii 0673 partial rDNA, AY791700; Candida boidinii 0680 partial rDNA, AY791694; Pichia sp. 159 partial rDNA, AY791699; aox0673, AY791698; aox0680, AY791697; aoxA, AY791695; aoxB, AY791696. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.1236