The effect of cyclo-DfKRG peptide immobilization on titanium on the adhesion and differentiation of human osteoprogenitor cells

This study takes place in the field of development of a bioactive surface of titanium alloys. In this paper, titanium was functionalized with cyclo-DfKRG peptide by coating or grafting using different anchors (thiol or phosphonate) as spacers between the surface and the peptide. Cell adhesion, and d...

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Bibliographic Details
Published in:Biomaterials 2005-12, Vol.26 (34), p.6932-6940
Main Authors: Pallu, Stéphane, Bourget, Chantal, Bareille, Reine, Labrugère, Christine, Dard, Michel, Sewing, Andreas, Jonczyk, Alfred, Vernizeau, Michel, Christine Durrieu, Marie, Amédée-Vilamitjana, Joelle
Format: Article
Language:English
Subjects:
Adhesion
Adsorption
Cell Adhesion - drug effects
Cell Adhesion - physiology
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cells, Cultured
Coated Materials, Biocompatible - chemistry
Coated Materials, Biocompatible - pharmacology
Cyclo-DfKRG peptide
Differentiation
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Hematopoietic Stem Cells - physiology
Humans
Materials Testing
Osteoblast
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - physiology
Osteogenesis - drug effects
Osteogenesis - physiology
Peptides, Cyclic - chemistry
Peptides, Cyclic - pharmacology
Protein Binding
Titanium - chemistry
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Summary:This study takes place in the field of development of a bioactive surface of titanium alloys. In this paper, titanium was functionalized with cyclo-DfKRG peptide by coating or grafting using different anchors (thiol or phosphonate) as spacers between the surface and the peptide. Cell adhesion, and differentiation of human osteoprogenitor (HOP) cells arising from human bone marrow were investigated. Our results seem to demonstrate that cyclo-DfKRG peptide coating with a phosphonate anchor and grafting procedure contributes to higher cell adhesion and a strong ALP and Cbfa1 mRNA expression, after 10 days of cell seeding. At the contrary, this peptide coated with a thiol anchor stimulates differentiation of HOP within 3 days of culture.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2005.04.054